July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Autophagy and mitophagy in SLC4a11 KO Corneal Endothelial cells
Author Affiliations & Notes
  • Raji Shyam
    Optometry, Indiana University Bloomington, Bloomington, Indiana, United States
  • Diego Gabriel Ogando
    Optometry, Indiana University Bloomington, Bloomington, Indiana, United States
  • Moonjung Choi
    Optometry, Indiana University Bloomington, Bloomington, Indiana, United States
  • Joseph A Bonanno
    Optometry, Indiana University Bloomington, Bloomington, Indiana, United States
  • Footnotes
    Commercial Relationships   Raji Shyam, None; Diego Ogando, None; Moonjung Choi, None; Joseph Bonanno, None
  • Footnotes
    Support  Indiana Clinical and Translational Sciences Institute (TL1 Program), TL1 TR002531 (A. Shekhar, PI).
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 2160. doi:
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    • Get Citation

      Raji Shyam, Diego Gabriel Ogando, Moonjung Choi, Joseph A Bonanno; Autophagy and mitophagy in SLC4a11 KO Corneal Endothelial cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):2160.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The goal of this study is to understand how loss of the membrane protein SLC4A11 alters endothelial cell metabolism thereby producing Corneal Endothelial Dystrophy. Studies from our lab indicated that glutamine-dependent mitochondrial dysfunction is one of the outcomes of SLC4a11 loss. In the current study, we ask if autophagy and mitophagy pathways and the signaling pathways that regulate these processes are altered in SLC4a11 KO cells.

Methods : Immortalized mouse WT and SLC4a11 KO cell lines were incubated in DMEM with and without 0.5mM glutamine for 6 hours. In order to assess mitophagy, cells were stained using Lysotracker Red and Mitotracker Green. Colocalization co-efficients of red and green channels were obtained for at least 35 cells using Zeiss-Zen Pro software. Student’s t-test was used to determine statistical significance. For Western Blots, antibodies against LC3b, AMPK, pAMPK, and b-actin were used to examine autophagy flux and potential signaling pathways that regulate autophagy.

Results : In the presence of glutamine, the colocalization co-efficient of Lysotracker Red and Mitotracker Green channels was significantly increased in KO cells (0.74 ±0.18) relative to WT (0.58±0.20) with a p-value ≤0.0024. In the absence of glutamine, the colocalization co-efficient was reversed, for KO cells 0.54 ±0.14 and for WT cells 0.77±0.0.16 with a p-value ≤0.0001, suggesting increased mitophagy by glutamine in KO cells. Western Blots indicated that glutamine increased autophagy flux, as indicated by increased levels of LC3b following bafilomycin A treatment in KO cells. Concomitantly, there was an increase in pAMPK/AMPK levels suggesting a potential mechanism for increased mitophagy.

Conclusions : Our data indicates enhanced mitophagy as well as autophagy in SLC4a11 KO cells. Future studies will determine whether these processes regulate cell survival in mouse models of corneal endothelial dystrophies.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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