Abstract
Purpose :
Determination of bacterial surface adhesion to various hydrophilic and hydrophobic IOL models.
Methods :
A quantitative, standardized method for investigating the bacterial adhesion and colonization of IOL surfaces by two different Staphylococcus epidermidis strains was used. The lenses were incubated for 3 hours at 37°C in a bacterial suspension at a concentration of 1 x 10^7 CFU/ml. They were then washed thoroughly with phosphate buffer solution (PBS) and exposed to an ultrasonic field of 75 kHz in a waterbath for three minutes. Subsequently, 50 µl of the suspension with the bacteria detached by ultrasound were plated out on a blood agar plate. The number of living bacteria was determined by the number of colonies growing.
Results :
13 IOL types from 3 different groups were used: hydrophobic group (SN60WF [Alcon], Sensar, Tecnis [AMO], enVista MX60P [B&L], iSert 251, Vivinex XY1 [HOYA], Avansee PU6A [Kowa], Aktis SP NS60YG [Nidek]; CT Lucia [Zeiss]), hydrophilic-hydrophobic group (mini-well [Sifi]) and hydrophilic group (C-Flex 570C, Sulcoflex 653L [Rayner]; CT Asphina [Zeiss]). The average colonization of S. epidermidis (ATCC 35984) was higher in the hydrophilic (128.284±12.561 CFU/ml) than in the hydrophobic (75.382±15.347 CFU/ml) and hydrophilic-hydrophobic (116.007±79.649 CFU/ml) group whereas the binding of S. epidermidis (patient strain from the eyeclinic) was higher in the hydrophilic-hydrophobic (148.666±71.127 CFU/ml) and hydrophobic (66.006±48.652 CFU/ml) than in the hydrophilic (42.341±47.046 CFU/ml) group. In general, S. epidermidis adhesion varied significantly on different hydrophilic as well as hydrophobic types of IOLs.
Conclusions :
There was significant variability of S. epidermidis adhesion to IOLs depending on design, material and surface modifications. The application of the results may lead to new developments to further reduce infectious endophthalmitis, which is still the one major complication in cataract surgery.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.