July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Dendritic cells induce Th17 inflammation in Aspergillus fumigatus keratitis
Author Affiliations & Notes
  • Xinyi Wu
    Ophthal QiLu Hosp/Ophthal, Shandong University , Jinan, Shandong , China
  • Footnotes
    Commercial Relationships   Xinyi Wu, None
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Investigative Ophthalmology & Visual Science July 2019, Vol.60, 2546. doi:
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      Xinyi Wu; Dendritic cells induce Th17 inflammation in Aspergillus fumigatus keratitis. Invest. Ophthalmol. Vis. Sci. 2019;60(9):2546.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : To investigate therole ofdendritic cellsinthe induction of Th17 inflammationin Aspergillus fumigatus keratitis.

Methods : After structuring the C57 mice animal model of Aspergillus fumigatus keratitis, examined at different points of infection time by slit lamp photograph to observe the degree of infection and clinical outcomes by clinical score. Getting the corneal tissues during blank and different infection time to detect the infiltration of inflammatory cells, the expression, distribution, migration, activation and typing of DCs,to detect the expressionof Th17 chemokines and the expression of Th17 cytokines secreted by Th17 cells.

Results : In this study, the infection of animal model became gradually aggravated with the course of infection time, reaching the peak on the third day after infection, then gradually subsiding, and self-healed on the seventh day after infection.PCR, Elisa and other tests showed that the expression ofDCs increased significantly during the infection.DCs aggregated, matured, and gradually migratedfrom the basement membrane to the corneal epithelium.During this process, the expression of Th17 chemokine in cornea increased significantly, and the amount of Th17 cytokine secreted by Th17 cells also increased significantly.

Conclusions : We demonstrated that in A.fumigatus keratitis, DC aggregated, maturated, and migrated, promoting the secretion of Th17-attracting chemokines, inducing the secretion of Th17 cytokines, which finally induced Th17 inflammation.In this process, inflammatory cells such as MΦ, PMN and NK cells also participate in the immune response inAspergillus fumigatus keratitis. The result presents a theoretical base for researching the corneal immune defense system and searching the effective way to confront fungal infection.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.


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