July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Ranibizumab prevents Müller cell edema in diabetic retinopathy
Author Affiliations & Notes
  • Jingfa Zhang
    Department of Ophthalmology, Renji Hospital, Shanghai, China
  • Tianqin Wang
    Department of Ophthalmology, Renji Hospital, Shanghai, China
  • Chaoyang Zhang
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, China
  • Hai xie
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, China
  • Qiuxue Yi
    Department of Ophthalmology, Renji Hospital, Shanghai, China
  • Dandan Liu
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, China
  • Qing Peng
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, China
  • Weiye Li
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, China
    Department of Ophthalmology, Drexel University College of Medicine, Pennsylvania, United States
  • Guo-Tong Xu
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, China
  • Lin Liu
    Department of Ophthalmology, Renji Hospital, Shanghai, China
  • Footnotes
    Commercial Relationships   Jingfa Zhang, None; Tianqin Wang, None; Chaoyang Zhang, None; Hai xie, None; Qiuxue Yi, None; Dandan Liu, None; Qing Peng, None; Weiye Li, None; Guo-Tong Xu, None; Lin Liu, None
  • Footnotes
    Support  This work was supported by National Natural Science Foundation of China (81570852) and Renji Hospital (PYIII-17-030).
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 2653. doi:
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      Jingfa Zhang, Tianqin Wang, Chaoyang Zhang, Hai xie, Qiuxue Yi, Dandan Liu, Qing Peng, Weiye Li, Guo-Tong Xu, Lin Liu; Ranibizumab prevents Müller cell edema in diabetic retinopathy. Invest. Ophthalmol. Vis. Sci. 2019;60(9):2653.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To explore the mechanism(s) of ranibizumab targeting Müller glia for intracellular edema in diabetic retinopathy.

Methods : Sprague-Dawley rats were rendered diabetes with intraperitoneal injection of streptozotocin. Intravitreal injection of ranibizumab (20 mg/eye) was performed 8 weeks after diabetes onset. Four weeks later, the rats were sacrificed and the retinas were harvested for detection. rMC-1 cells were treated with glyoxal (1 mM) for 24 hours, with or without ranibizumab. Cell viability was detected with CCK-8 assay. The expressions of Kir4.1, Dp71, VEGF-A and Glutamine synthetase were examined with WB. VEGF-A in the supernatant was detected with ELISA. The intracellular potassium and sodium level was detected with the specific probe.

Results : Compared to the normal control, the protein expressions of Kir4.1 and Dp71 were down-regulated significantly in diabetic rat retina, which could be reversed by ranibizumab. The above changes were confirmed with in vitro study. The intracellular potassium level is increased in glyoxal-treated rMC-1 cells compared with that in normal control, while intracellular sodium level, but not potassium, was decreased by ranibizumab.

Conclusions : Ranibizumab, through binding VEGF-A, could maintain the expressions of Kir4.1 and Dp71 to decrease the intracellular osmotic pressure, thus protect Müller cells from swelling.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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