Abstract
Purpose :
Diabetic microvascular complications is the main cause of death and disability in diabetes. Diabetic retinopathy(DR) and diabetic neuropathy (ND) are the main microvascular complications of diabetes, representing the leading cause of blindness and end-stage renal diseases. The aims of the study are to investigate the related key regulatory genes, the potential target and predict biomarkers for both DR and DN.
Methods :
225 blood samples were collected from patients with diabetes after the informed consent form were signed. The related key regulatory genes between DR and DN was investigated by miRNA chip. The relationship of the pathogenesis between DR/DN was further elucidated using GEO database and Network-based meta analysis.The target genes were further elucidated by Venn diagram from the GO/network analysis and KEGG pathways.
Results :
1326 genes have been identified for the pathogenesis of DR and DN.33 genes are the common pathogenesis genes for both DR and DN, among which 6 genes have strong regulatory relationship in the pathogenesis of DR and DN. The vascular leakage related pathway: VEGF-VEGFR-miRNA 126-APRED-Ras signalling pathway and neuronal apoptosis related pathway: P38MAPK-CASPASE-3-PARP-CytC and miRNA200b-OXr-1 signalling pathways were found to be involved in the pathogenesis of DR and DN. miR-21, miR-192, miR-200, miR-29A may be DN, miR-146 may be the specific markers and molecular targets for both DR and DN.
Conclusions :
Genes, mRNA and miRNA constitute a regulatory network in the pathogenesis of DR and DN. Microvascular leakage and neuronal apoptosis plays an important role in the pathological process of DR and DN. Discovery of molecular targets is the prerequisite and basis for achieving precise treatment.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.