July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Lacking SIPr3 attenuate the growth of Argon laser-induced choroidal neovascularization.
Author Affiliations & Notes
  • HIroki Iwanishi
    Opthalmology, Wakayama Medecal University, Wakayama City, Wakayama, Japan
  • Shingo Yasuda
    Opthalmology, Wakayama Medecal University, Wakayama City, Wakayama, Japan
  • Takayoshi Sumioka
    Opthalmology, Wakayama Medecal University, Wakayama City, Wakayama, Japan
  • Yuka Okada
    Opthalmology, Wakayama Medecal University, Wakayama City, Wakayama, Japan
  • Masayasu Miyajima
    Opthalmology, Wakayama Medecal University, Wakayama City, Wakayama, Japan
  • Kana Ichikawa
    Opthalmology, Wakayama Medecal University, Wakayama City, Wakayama, Japan
  • Shizuya Saika
    Opthalmology, Wakayama Medecal University, Wakayama City, Wakayama, Japan
  • Footnotes
    Commercial Relationships   HIroki Iwanishi, None; Shingo Yasuda, None; Takayoshi Sumioka, None; Yuka Okada, None; Masayasu Miyajima, None; Kana Ichikawa, None; Shizuya Saika, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 2979. doi:https://doi.org/
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      HIroki Iwanishi, Shingo Yasuda, Takayoshi Sumioka, Yuka Okada, Masayasu Miyajima, Kana Ichikawa, Shizuya Saika; Lacking SIPr3 attenuate the growth of Argon laser-induced choroidal neovascularization.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):2979. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the effects of lacking sphingosine-1-phosphate receptor type 3 (SIPr3) on the development of experimental choroidal neovascularization (CNV) induced by ocular fundus Argon-laser irradiation in mice.

Methods : Male C57BL/6 wild-type (WT) and C57BL/6-background SIPr3-null (KO) mice of 6 - 8 week-old were used. (1) Argon laser-photocoagulation (5 shots, 80 m spot size, 0.05 sec. duration, 200 mW) was performed in the ocular fundus of WT (n = 5) and KO (n = 5) mice. Mice received FITC-labeled dextran angiography and the size of CNV was evaluated in the flat-mounted retino-choroidal tissue at day 14 with computer software assistance (2) Laser photocoagulation (25 shots) was performed in the eye of WT (n = 20) and KO (n = 20) mice. Total RNA was extracted from retino-choroidal tissue at day 1 and 3. Real-time RT-PCR was ran for mRNAs of VEGF-A, IL-6, MCP-1, TGFb1, MPO (neutrophil marker), F4/80 (macrophage marker) and aSMA.

Results : (1) The size of laser-induced CNV formation was significantly smaller in KO mice at day 14 than in WT mice (p < 0.05). (2) The loss of SIPr3 suppressed mRNA expression of TGFb1, MPO, F4/80 (at day 1) and MCP-1 (at day 1 and day 3) in retino-choroidal tissue post laser irradiation (p < 0.05).

Conclusions : SIPr3 deficiency attenuates the growth of laser-induced CNV in association with suppression of inflammation.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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