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HIroki Iwanishi, Shingo Yasuda, Takayoshi Sumioka, Yuka Okada, Masayasu Miyajima, Kana Ichikawa, Shizuya Saika; Lacking SIPr3 attenuate the growth of Argon laser-induced choroidal neovascularization.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):2979.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effects of lacking sphingosine-1-phosphate receptor type 3 (SIPr3) on the development of experimental choroidal neovascularization (CNV) induced by ocular fundus Argon-laser irradiation in mice.
Male C57BL/6 wild-type (WT) and C57BL/6-background SIPr3-null (KO) mice of 6 - 8 week-old were used. (1) Argon laser-photocoagulation (5 shots, 80 m spot size, 0.05 sec. duration, 200 mW) was performed in the ocular fundus of WT (n = 5) and KO (n = 5) mice. Mice received FITC-labeled dextran angiography and the size of CNV was evaluated in the flat-mounted retino-choroidal tissue at day 14 with computer software assistance (2) Laser photocoagulation (25 shots) was performed in the eye of WT (n = 20) and KO (n = 20) mice. Total RNA was extracted from retino-choroidal tissue at day 1 and 3. Real-time RT-PCR was ran for mRNAs of VEGF-A, IL-6, MCP-1, TGFb1, MPO (neutrophil marker), F4/80 (macrophage marker) and aSMA.
(1) The size of laser-induced CNV formation was significantly smaller in KO mice at day 14 than in WT mice (p < 0.05). (2) The loss of SIPr3 suppressed mRNA expression of TGFb1, MPO, F4/80 (at day 1) and MCP-1 (at day 1 and day 3) in retino-choroidal tissue post laser irradiation (p < 0.05).
SIPr3 deficiency attenuates the growth of laser-induced CNV in association with suppression of inflammation.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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