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hajime takahashi, Kazuki Nakagawa, Haruhiko Yamada, Hidetsugu Mori, Kanji Takahashi; Optical Coherence Tomography Angiography in laser-induced mouse branch retinal vein occulusion model. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3073.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the ability of optical coherence tomography angiography (OCTA), we investigate the stuructural change of retinal vessels in laser-induced mouse branch retinal vein obstruction(BRVO) model.
Under deep anesthesia, 9 BALB/C female mice aged 6 weeks were injected 0.75mg rose bengal dye intraperitoneously. Immediately after that, they received laser irradiation (GYC-2000, NIDEK; wavelength 532nm) in order to stop blood flow of retinal vein. As for the laser, single vein which had 1-2 optic nerve diameter apart from the optic nerve head was selected and irradiated by power of 100mW, 50μm diameter, and 3 seconds duration. OCTA (RS-3000 Advance, NIDEK) was used to eveluate retinal vascular changes and observe blood flow before and 1,3,5,7,14 days after laser.
Vein occlusion was succesfully achieved in 7 out of 9 eyes. In 6 eyes with vein occlusion, collateral blood vessels were found and they flowed into another vein from the distal point of occluded spot at day 3 and day 5 after laser. In one eye, the vein that occluded at day 5 after laser became reperfused, and accordingly collateral blood vessel disappeared in OCTA image. The rest of 5 eyes, the collateral vessels maintained through 14 days after laser.
Gradual changes of retinal vascular structure in the experimental BRVO mouse model could be observed by using OCTA. OCTA was useful because it could repeatedly capture changes of the retinal vascular structure in lower penetration to the animal compared to conventional angiography.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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