July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Establishment of a hypoxia model for the MEA-based analysis of electrical activity of murine retina
Author Affiliations & Notes
  • Claudia Ingensiep
    Department of Ophthalmology, University Hospital RWTH Aachen, Aachen, Germany
  • Kim Schaffrath
    Department of Ophthalmology, University Hospital RWTH Aachen, Aachen, Germany
  • Peter Walter
    Department of Ophthalmology, University Hospital RWTH Aachen, Aachen, Germany
  • Sandra Johnen
    Department of Ophthalmology, University Hospital RWTH Aachen, Aachen, Germany
  • Footnotes
    Commercial Relationships   Claudia Ingensiep, None; Kim Schaffrath, None; Peter Walter, None; Sandra Johnen, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3099. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Claudia Ingensiep, Kim Schaffrath, Peter Walter, Sandra Johnen; Establishment of a hypoxia model for the MEA-based analysis of electrical activity of murine retina. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3099.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Several eye diseases, e.g. retinal artery occlusion, diabetic retinopathy or glaucoma, are associated with retinal hypoxia (ischemia). The lack of oxygen in the retina, especially in retinal ganglion cells (RGCs), causes cell damage up to cell degeneration leading to blindness. To examine the activity of RGCs and analyze the effect of neuroprotective substances under hypoxic conditions is of great ophthalmologic interest.

Methods : Using multielectrode array (MEA) recordings, two ex vivo models were established to analyze the electrical activity of murine wild-type (wt) retina under hypoxic stress conditions. In model 1, hypoxic conditions were initiated by stopping the perfusion with oxygen saturated medium. In model 2, hypoxia was induced by exchanging the perfusion with oxygen saturated medium by nitrogen saturated medium, which allowed for more precisely defined test conditions. To analyze the influence of neuroprotective agents on the firing behavior of RGCs under hypoxia-induced conditions, 2-aminoethanesulfonic acid (taurine, 1 mM) was added during a hypoxia time of 30 min.

Results : In both models, the electrical activity vanished during hypoxia. However, it conditionally returned after reestablishment of the conventional test conditions. With increasing duration of hypoxia, the number of recording channels, on which activity could be redetected, decreased. After a hypoxic period of 30 min and a subsequent recovery time of 30 min, 52.14 ± 26.69 % of the initially active channels showed a restored activity within the first model, and 59.43 ± 11.35 % within the second. The application of taurine had a positive influence on the electrical excitability of RGCs. However, regarding the number of recording channels and the firing frequency of the spontaneous activity after hypoxia, no positive effects were observed.

Conclusions : The hypoxia models established here allow for the analysis of electrical RGC activity before, during and after hypoxic conditions as well as the investigation of the effect of any protective substance on the retinal electric activity. Further experiments regarding the analysis of the RGC excitability after light stimulation are ongoing. For closer investigation of the effect of taurine, a microarray-based transcriptome-wide gene expression analysis of the retina has been performed using different culture conditions.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×