July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Light exposure history alters dopamine activity in the retina
Author Affiliations & Notes
  • Erica Landis
    Atlanta VA Health System, Georgia, United States
  • Han na Park
    Ophthalmology, Emory University School of Medicine, Georgia, United States
  • Micah A Chrenek
    Ophthalmology, Emory University School of Medicine, Georgia, United States
  • Li He
    Ophthalmology, Emory University School of Medicine, Georgia, United States
  • Curran Sidhu
    Ophthalmology, Emory University School of Medicine, Georgia, United States
  • Ryan Strickland
    Atlanta VA Health System, Georgia, United States
  • P. Michael Iuvone
    Ophthalmology, Emory University School of Medicine, Georgia, United States
    Pharmacology, Emory University School of Medicine, Georgia, United States
  • Machelle T Pardue
    Biomedical Engineering, Georgia Institute of Technology, Georgia, United States
    Atlanta VA Health System, Georgia, United States
  • Footnotes
    Commercial Relationships   Erica Landis, None; Han na Park, None; Micah Chrenek, None; Li He, None; Curran Sidhu, None; Ryan Strickland, None; P. Michael Iuvone, None; Machelle Pardue, None
  • Footnotes
    Support  NIH NEI R01 EY016435, R01 EY004864, P30 EY006360, and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3152. doi:
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    • Get Citation

      Erica Landis, Han na Park, Micah A Chrenek, Li He, Curran Sidhu, Ryan Strickland, P. Michael Iuvone, Machelle T Pardue; Light exposure history alters dopamine activity in the retina. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3152.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Ambient light environments alter myopia susceptibility. We previously showed that photopic (15,000 lux) or scotopic (0.005 lux), but not mesopic (50 lux), ambient lighting protected mice from lens-induced myopia (ARVO 2015 E-Abstract #2152). Here we investigate the role of ambient light level on dopamine (DA) activity underlying the altered myopia susceptibility by examining DA activity after acute or long-term light exposure.

Methods : Male C57BL/6J mice were placed in one of three illuminance conditions (photopic n=21, mesopic n=22, scotopic n=22) on a 12:12 L:D cycle beginning at post-natal day 23 (P23). Retinas were collected at three hours (acute) or twelve days (long-term). Levels of DA and DOPAC in the retinas were measured with HPLC. Digital droplet PCR and western blots were used to detect changes in gene expression and protein levels related to dopamine synthesis (tyrosine hydroxylase, TH), release (vesicular monoamine transporter 2), uptake (dopamine transporter), and degradation (monoamine oxidase). Results were analyzed using two-way ANOVA with Holm-Sidak post-hoc comparisons.

Results : After acute light exposure, retinas in scotopic light showed significantly higher levels of DA (1.11 normalized to mesopic, p<0.01) and lower levels of DOPAC (0.48 normalized to mesopic, p<0.001) than mice housed in mesopic and photopic (DA: 0.86, DOPAC: 1.48 both normalized to mesopic). With long-term light exposure, differences in DA levels stabilized across groups. DOPAC was still lower in scotopic and higher in photopic conditions compared to mesopic light (p<0.001). An analysis of mRNA levels showed increasing expression of TH with increasing illuminance after long-term light but not acute exposure. Protein levels did not change with illuminance or duration of exposure.

Conclusions : These results suggest that changes in dopamine dynamics with ambient lighting conditions are influenced by light exposure history. While retinal DA levels were stable across illuminance levels with long-term exposure, the different DA levels after acute exposure may indicate that the retina produces DA in anticipation of a particular light level based on light exposure history. While these results would suggest that higher DA activity under photopic lighting mediates the protective effects on lens-induced myopia, further research is needed to determine whether DA activity underlies the protective effects under scotopic lighting.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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