Abstract
Purpose :
To determine what provisional matrix components are expressed after ocular nitrogen mustard injury
Methods :
corneal organ culture, Western analyses, histology
Results :
The provisional matrix deposited in corneal organ cultures was followed for 7 days after a 100 nM nitrogen mustard exposure for 60 min. By immunofluorescence analysis, corneas were found to express provisional matrix components hevin, SPARC, thrombospondin, and tenascin C within 1 day of exposure, then decrease over the course of the following 6 days. In contrast, by immunofluorescence analysis of fibronectin was weak at 1 day post exposure, and increased did not appear until 7 days after exposure. SPARC was highly expressed at 1 day post exposure by Western analysis, and gradually decreased over the 7 days. SPARC and hevin were both expressed highly within 1 day after exposure, while fibronectin expression was late by comparison, being initially seen as a slight band at 5 days after exposure, and was more abundant by Western analysis at 7 days post NM exposure.
Conclusions :
Nitrogen Mustard causes ocular injury that activates provisional matrix expression.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.