July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Deposition of Provisional Matrix Components in Corneas Exposed to Nitrogen Mustard Persists at Least 7 Days After Exposure
Author Affiliations & Notes
  • Marion K Gordon
    Pharmacology & Toxicology, Rutgers University, Piscataway, New Jersey, United States
  • Rita Hahn
    Pharmacology & Toxicology, Rutgers University, Piscataway, New Jersey, United States
  • Peihong Zhou
    Pharmacology & Toxicology, Rutgers University, Piscataway, New Jersey, United States
  • Yokechen Chang
    Pharmacology & Toxicology, Rutgers University, Piscataway, New Jersey, United States
  • Donald Gerecke
    Pharmacology & Toxicology, Rutgers University, Piscataway, New Jersey, United States
  • Footnotes
    Commercial Relationships   Marion Gordon, None; Rita Hahn, None; Peihong Zhou, None; Yokechen Chang, None; Donald Gerecke, None
  • Footnotes
    Support  NINDS AR055073
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3205. doi:
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      Marion K Gordon, Rita Hahn, Peihong Zhou, Yokechen Chang, Donald Gerecke; Deposition of Provisional Matrix Components in Corneas Exposed to Nitrogen Mustard Persists at Least 7 Days After Exposure. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3205.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine what provisional matrix components are expressed after ocular nitrogen mustard injury

Methods : corneal organ culture, Western analyses, histology

Results : The provisional matrix deposited in corneal organ cultures was followed for 7 days after a 100 nM nitrogen mustard exposure for 60 min. By immunofluorescence analysis, corneas were found to express provisional matrix components hevin, SPARC, thrombospondin, and tenascin C within 1 day of exposure, then decrease over the course of the following 6 days. In contrast, by immunofluorescence analysis of fibronectin was weak at 1 day post exposure, and increased did not appear until 7 days after exposure. SPARC was highly expressed at 1 day post exposure by Western analysis, and gradually decreased over the 7 days. SPARC and hevin were both expressed highly within 1 day after exposure, while fibronectin expression was late by comparison, being initially seen as a slight band at 5 days after exposure, and was more abundant by Western analysis at 7 days post NM exposure.

Conclusions : Nitrogen Mustard causes ocular injury that activates provisional matrix expression.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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