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Tatsuhiko Kobayashi, Takashi Suzuki, Takashi Itokawa, Koji Kakisu, Yuichi Hori; Comparison of leukocyte-rich platelet-rich plasma and pure platelet-rich plasma eye drops for release of growth factors. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3223.
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© ARVO (1962-2015); The Authors (2016-present)
To compare the effects of leukocyte-rich platelet-rich plasma (L-PRP) and pure platelet-rich plasma (P-PRP) eye drops for releasing the growth factors, platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF), with two goals: to investigate the function of leukocytes in PRP eye drops in corneal tissue repair and determine the effects of four methods of preservation of the eye drops regarding the release of growth factors.
L-PRP and P-PRP eye drops were extracted from 30 millimeters of whole blood obtained from healthy adults using the TriCeLL PRP kit (Yamato Scientific Co., Ltd.) and the double-spin method, respectively. After the numbers of blood cells in the L-PRP and P-PRP eye drops were counted, they were preserved using four methods: 1-hour storage at room temperature, 1-hour storage at -20 °C, 24-hour storage at 4°C, and 24-hour storage at -20°C. PDGF-BB, PDGF-AB, EGF, and VEGF were measured by ELISA in the L-PRP and P-PRP eye drops.
Four milliliters of L-PRP and P-PRP were extracted from the whole blood samples. The following blood cells were identified in the L-PRP eye drops: platelets, 60.8±172.2×104 and white blood cells, 10.4±14.7x103; and in the P-PRP eye drops, platelets, 35.5±94.0×104 and white blood cells, 0.5±0.2x103. The concentrations of PDGF (pg/ml) in the L-PRP eye drops based on the four storage methods were, respectively, PDGF-BB, 89.3±16.2, 235.6±132.2, 250.9±170.6, and 521.7±207.3; and PDGF-AB, 32.6±11.5, 121.5±44.9, 165.3±46.5, and 334.6±15.3, all of which were higher than in the P-PRP eye drops: PDGF-BB, 70.2±3.4, 122.8±48.0, 150.0±97.0, and 254.7±74.2; and PDGF-AB, 15.6±8.9, 53.6±16.1, 98.0±42.8, and 159.9±28.3. The concentrations of EGF and VEGF in the both eye drops were similar.
Freezing and thawing of PRP eye drop may include high concentrations of PDGF, and leukocytes may increase PDGF release from the platelets.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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