July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Expression of lubricin in the human amniotic membrane
Author Affiliations & Notes
  • Jingyi Wang
    Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, HMS, Massachusetts, United States
    Ophthalmology, Peking Union Medical College Hospital, Beijing, China
  • David A Sullivan
    Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, HMS, Massachusetts, United States
  • Di Chen
    Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, HMS, Massachusetts, United States
    Ophthalmology, Peking Union Medical College Hospital, Beijing, China
  • Huatao Xie
    Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, HMS, Massachusetts, United States
    Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China
  • Ying Li
    Ophthalmology, Peking Union Medical College Hospital, Beijing, China
  • Yang Liu
    Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, HMS, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Jingyi Wang, None; David Sullivan, Lµbris BioPharma (I), Schepens Eye Research Institute/Massachusetts Eye and Ear Infirmary (P); Di Chen, Schepens Eye Research Institute/Massachusetts Eye and Ear Infirmary (P); Huatao Xie, None; Ying Li, None; Yang Liu, Schepens Eye Research Institute/Massachusetts Eye and Ear Infirmary (P)
  • Footnotes
    Support  The Margaret S. Sinon Scholar in Ocular Surface Research fund, the One-Hundred-Talent Scholarship Program of Peking Union Medical College Hospital, China Scholarship Council, and NIH National Eye Institute Core Grant P30EY003790
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3232. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jingyi Wang, David A Sullivan, Di Chen, Huatao Xie, Ying Li, Yang Liu; Expression of lubricin in the human amniotic membrane. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3232.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Lubricin is the body’s unique anti-adhesive, anti-fibrotic, anti-friction and anti-inflammatory glycoprotein. It is produced by numerous tissues, including the cornea, heart, lung, liver, cartilage, kidney, brain, testis, placenta and small intestine. At these locations lubricin may regulate a number of processes, such as homeostasis, shear stress, tissue development, inflammation and wound healing. We hypothesize that lubricin is also synthesized and expressed by the amniotic membrane (AM). Our rationale is that the AM possesses anti-adhesive, anti-fibrotic and anti-inflammatory properties, and promotes tissue redevelopment and wound healing. We believe that lubricin, at least in part, mediates these AM capabilities.

Methods : We obtained ten de-identified samples of human AMs and positive control placentas from the Tissue Repository of the Massachusetts General Hospital Pathology Service (Boston). These tissues originated from healthy donors (29 to 38 years old) following Cesarean sections. Our studies were approved by the Human Studies Committee of the Massachusetts Eye and Ear Infirmary (Boston). We also obtained one cryopreservated (CP-AM) and five freeze-dried (FD-AM) AMs. The FD-AMs were evaluated with or without incubation overnight at 4°C with recombinant human (rh) lubricin (50 µl, 0.675 mg/ml, gift from Lµbris BioPharma, Framingham, MA). Samples were processed for immunofluorescence and Western blot analyses.

Results : Our results demonstrate that all placental and AM samples contained lubricin. Lubricin was expressed in placental chorionic villi and AM epithelial and stromal cells. Lubricin was also present in CP-AM epithelia, but not in the FD-AMs. Lubricin expression could be restored in FD-AM after overnight incubation with rhlubricin.

Conclusions : This study demonstrates that lubricin is expressed in human AMs, and that preservation methods influence the extent of this expression. Given lubricin's biological activities, it is possible that this glycoprotein may mediate, at least in part, the anti-adhesive, anti-fibrotic and anti-inflammatory properties of AMs.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×