July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
The effect of topical NGF, EGF, IGF-1 administration on healing of corneal epithelial defect in a mouse model of neurotrophic keratopathy
Author Affiliations & Notes
  • Yuka Okada
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Shizuya Saika
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Footnotes
    Commercial Relationships   Yuka Okada, None; Shizuya Saika, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3243. doi:https://doi.org/
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      Yuka Okada, Shizuya Saika; The effect of topical NGF, EGF, IGF-1 administration on healing of corneal epithelial defect in a mouse model of neurotrophic keratopathy. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3243. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We examined if either, NGF, EGF, or IGF-1, exerts therapeutic effects of acceleration of epithelial wound healing in a neurotrophic keratopathy mouse model when topically applied. We previously reported delayed corneal epithelial wound healing in this model (Okada et al. Lab invest – on line-, 2018).

Methods : Six to 8 week-old male C57BL/6 mice (n = 20) underwent stereotactic trigeminal coagulation to destroy the ophthalmic branch (V1) of right trigeminal nerve as previously reported (Okada et al. Lab invest – on line-, 2018). In this model corneal epithelium did not breakdown without external insult, but showed impaired epithelial healing after producing a defect. Three months after denervation, wound healing of central epithelial defect in cornea was evaluated at 6 to 30 hrs after epithelial ablation with NGF (200 μg/ml), EGF (10 μ/ml), IGF-1 (10 μg/ml) or PBS eye drops each 6 hrs. The proliferation activity of the healing epithelium was determined by using BrdU-immunostaining at 30 hrs.

Results : Topical NGF administration reversed the impairment of epithelial healing by V1 denervation with statistical significance at 12 and 18hrs. Topical IGF-1 also reversed the attenuation of epithelial healing at 30hrs. However, EGF application had no effect on the corneal wound healing in this mouse model. NGF and EGF eye drops did not affect cell proliferation activity in corneal epithelium after epithelial defect. While BrdU-positive epithelial cells were increased in IGF-1-group as compared to control PBS.

Conclusions : Topical NGF and IGF-1 exhibited therapeutic effects on a mouse model of neurotrophic keratopathy.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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