July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Enrichment and characterization of self-forming RPE Spheroids concurrently generated with Retinal Organoids from hiPSCs
Author Affiliations & Notes
  • Xiufeng Zhong
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Shengxu Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Bingbing Xie
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • xiaojing song
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Dandan Zheng
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Liwen HE
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Guanjie Gao
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Footnotes
    Commercial Relationships   Xiufeng Zhong, None; Shengxu Liu, None; Bingbing Xie, None; xiaojing song, None; Dandan Zheng, None; Liwen HE, None; Guanjie Gao, None
  • Footnotes
    Support  This study was supported by the National Key R&D Program of China (2017YFA0104100; 2016YFC1101103), the National Natural Science Foundation of China (81570874), the Science & Technology Project of Guangdong Province (2017B020230003; 2014A020211008; 2015A020212011), the Science & Technology Project of Guangzhou (201803010078), the Fundamental Research Funds for the Central Universities, the Hundred talent program of Sun Yat-sen University, the Fundamental Research Funds of the State Key Laboratory of Ophthalmology, the Zhongshan Ophthalmic Center fund.
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3323. doi:
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    • Get Citation

      Xiufeng Zhong, Shengxu Liu, Bingbing Xie, xiaojing song, Dandan Zheng, Liwen HE, Guanjie Gao; Enrichment and characterization of self-forming RPE Spheroids concurrently generated with Retinal Organoids from hiPSCs. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3323.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To enrich and expand hiPSC-derived retinal pigment epithelium (RPE) concurrently generated with neural retina on retinal organoid induction platform

Methods : hiPSCs were cultured on MatriGel coated plates with mTeSR1 medium, and differentiated into RPE cells with a published retinal organoid induction method. After detachment of neural retina on 4th wk, the remaining mixture was also scraped off from the dish and subjected to suspension culture for the formation of RPE spheroids. RPE cells were isolated for expansion. Different assays were used to evaluate the biological charateristics of RPE cells.

Results : Under suspension culture, hiPSC-RPE spheroids with pigmentation self-formed, and were readily enriched by removing out the non-retinal tissues. RPE cells could be readily purified from the spheroids, cultured and serially passaged in serum medium, yielding large numbers of cells with high quality in a short period. When switched to serum-free medium, the passaged RPE cells could mature in cellular, molecular and physiological levels.

Conclusions : A simple and novel RPE spheroids formation approach was established to enrich and expand hiPSC-RPE cells generated along with retinal neurons on a universal retinal organoid induction platform. This achievement will evidently reduce the cost and time in producing retinal cells for basic and translational researches, for retinal cell therapy in particular.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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