Abstract
Purpose :
Ocular health assessment supporting standard preclinical ocular examinations has generated interest in ocular toxicology testing. Few studies have quantified aqueous and vitreous humor electrolyte contents across species. This study aimed to quantify electrolytes in aqueous and vitreous humor from four preclinical species.
Methods :
Aqueous and vitreous humors were collected from rabbits (n=7), dogs (n=9), monkeys (n=10) and minipigs (n=7). Vitreous and aqueous humors were analyzed by Cobas®6000-c501 (plasma/serum mode). Electrolyte concentrations (mmol/L) in aqueous and vitreous humor were comparable between species.
Results :
Vitreous electrolyte concentrations were: phosphorus (0.27±0.02 to 0.33±0.19), calcium (1.41±0.18 to 2.34±0.25); sodium (132.80±16.52 to 159.00±12.16); potassium (4.40±0.27 to 5.30±0.36); chloride (99.40±14.53 to 123.90±9.57). Aqueous electrolyte concentrations were: phosphorus (0.36±0.04 to 0.90±0.08); calcium (1.25±0.10 to 2.18±0.04); sodium (142.00±6.11 to 153.67±4.58); potassium (3.78±0.11 to 5.26±0.99); chloride (97.80±4.07 to 116.40±2.97). Humor concentrations of sodium, potassium and chloride were generally similar to species-specific serum levels; however, phosphorus and calcium concentrations were slightly below serum levels.
Conclusions :
This study provided a baseline/reference of humor electrolytes. Electrolyte measurement from aqueous and vitreous humor could be a useful parameter in the assessment of ocular toxicity in toxicology studies. Electrolyte composition of these anatomical segments may also have implications relative to test compound chemistry after intra-ocular delivery.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.