July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
m6A RNA Modification Regulates Corneal Epithelial Wound Healing
Author Affiliations & Notes
  • Guangying Luo
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Yunping Zhao
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Peter S. Reinach
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Dongsheng Yan
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Footnotes
    Commercial Relationships   Guangying Luo, None; Yunping Zhao, None; Peter S. Reinach, None; Dongsheng Yan, None
  • Footnotes
    Support  National Natural Science Foundation of China (81700801); Foundation of Wenzhou Science & Technology Bureau (Y20170199); and Institutional Grants (YNCX201605, KYQD141104) of Eye Hospital, Wenzhou Medical University
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3438. doi:
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    • Get Citation

      Guangying Luo, Yunping Zhao, Peter S. Reinach, Dongsheng Yan; m6A RNA Modification Regulates Corneal Epithelial Wound Healing. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3438.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : N6-methyladenosine (m6A) is the most abundant RNA modification involved in various biological processes. Corneal epithelial wound healing (CEWH) is essential for restoring corneal integrity and transparency after injury. However, it is unclear whether m6A RNA modification modulates CEWH. Here, we investigated the role of m6A RNA modification in CEWH.

Methods : Dot blot and global m6A assays determined m6A RNA modification levels during mouse CEWH. Cycloleucine (Cyc), an RNA m6A inhibitor, was used to explore the effects of m6A RNA modification on CEWH by monitoring corneal fluorescein staining. MTS and scratch wound assay evaluated the effects of Cyc on human corneal epithelial cell (HCEC) proliferation and migration. Western blot detected the expression levels of cell proliferation and migration related proteins in Cyc treated HCECs.

Results : Global m6A RNA modification levels were significantly increased during mouse CEWH. Cyc markedly delayed CEWH in vivo. Furthermore, Cyc significantly inhibited HCEC proliferation and migration in vitro. In addition, Cyc decreased the protein levels of p-FAK and p-ERK1/2 in HCECs.

Conclusions : Our findings reveal a critical role of m6A RNA modification in modulating CEWH, which imply m6A as a novel epitranscriptomic marker in this process.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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