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miki onoue, Yosuke Asada, Satoshi Iwamoto, Toshiaki Hirakata, Akira Matsuda, KEISUKE ADACHI; The influence of macrophage depletion in mouse model of filtration surgery. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3754.
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© ARVO (1962-2015); The Authors (2016-present)
We have previously showed the activation of macrophage-related genes at day 3 using microarray analysis of mouse filtration surgery model (ARVO 2018). To clarify the roles of macrophage after filtration surgery, we analyzed mouse filtration surgery model under macrophage-depleted condition.The influence of macrophage depletion in mouse model of filtration surgery
Macrophage-depleted mouse models were made by the injection of 0.2 ml clodronate liposome into their tail vein on the day before filtration surgery. Filtration surgeries were carried out by the insertion of 30G needle tip into the anterior chamber and put the collagen sponge immersed with clodronate liposome into the subconjunctival space. The control models were made similarly using saline instead of clodronate liposome. The bleb tissues, sampled at day 3 after filtration surgery, were analyzed by immunohistochemical staining and quantitative PCR.
The bleb morphology at day 3 after filtration surgery had no significant differences between the macrophage-depleted mouse models and the controls. Immunohistochemical analysis of the bleb region showed greatly reduced CD68 positive macrophage in the macrophage-depleted mouse models. On the other hand, comparative numbers of Ly6G positive neutrophils were observed in both groups of the bleb region.Quantitative PCR analysis of the bleb region showed the significant attenuation of inflammatory cytokine genes (Il1b, Il6) and alpha-smooth muscle actin (Acta2) gene, in the macrophage-depleted mouse models compared to the control models.
Our results suggest that macrophage in the mouse models of filtration surgery are associated with the production of the inflammatory cytokines and the tissue fibrosis in the early phase after filtration surgery.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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