July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Utilization of scanning acoustic microscopy (SAM) in quantifying the biomechanical variations in tissues with Fuchs endothelial dystrophy (FECD)
Author Affiliations & Notes
  • Jean-Marc Perone
    ophthalmology department, Regional Hospital Center of Metz-Thionville, Mercy Hospital, Metz, France
  • Esam T. Ahmed Mohamed,
    Laboratory for Ultrasonic Nondestructive Evaluation ‘‘LUNE’’, UMI Georgia Tech–CNRS 2958, Metz, France
  • sebastian brand
    Center for Applied Microstructure Diagnostics CAM,, Fraunhofer Institute for Microstructure of Materials and Systems IMWS,, Halle, Germany
  • Michael Koegel
    Center for Applied Microstructure Diagnostics CAM,, Fraunhofer Institute for Microstructure of Materials and Systems IMWS,, Halle, Germany
  • Nico F. Declercq
    Laboratory for Ultrasonic Nondestructive Evaluation ‘‘LUNE’’, UMI Georgia Tech–CNRS 2958, Metz, France
  • Footnotes
    Commercial Relationships   Jean-Marc Perone, None; Esam T. Ahmed Mohamed,, None; sebastian brand, None; Michael Koegel, None; Nico F. Declercq, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3830. doi:
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      Jean-Marc Perone, Esam T. Ahmed Mohamed,, sebastian brand, Michael Koegel, Nico F. Declercq; Utilization of scanning acoustic microscopy (SAM) in quantifying the biomechanical variations in tissues with Fuchs endothelial dystrophy (FECD). Invest. Ophthalmol. Vis. Sci. 2019;60(9):3830.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The aim of this study was to demonstrate the application of Scanning Acoustic Microscopy in the GHz-range (GHz- SAM) to infer the biomechanical alterations in Descemet’s membrane and endothelial cells of cornea tissue with Fuchs endothelial corneal dystrophy (FECD).

Methods : Investigated were samples of a normal tissue and a tissue with Fuchs endothelial dystrophy (FECD, cornea Guttata). Descemet’s membranes were fixed on glass substrates and imaged utilizing a focused acoustic lens operating at a center frequency of 1 GHz.

Results : A discrepancy of 53±7 m/s between the velocity of Rayleigh surface acoustic waves (VRSAW) in glass substrates with Descemet’s membrane - FECD and VRSAW in glass with normal Descemet’s membrane were realized. Slower VRSAW in Descemet’s membranes with FECD indicates lower moduli of rigidity in membranes with FECD than in normal membranes.

Conclusions : Our data proofs SAM’s potential in quantifying the biomechanical variations of FECD relative to normal Descemet’s membranes based on the determination of velocity of propagation of ultrasound waves, which is related to the moduli of rigidity

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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