July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Surgical Placement and Degradation of Fibrin Scaffolds within the Subretinal Space of a Porcine Eye
Author Affiliations & Notes
  • Jarel K Gandhi
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Fukutaro Mano
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Stephen LoBue
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Timothy W Olsen
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Raymond Iezzi
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Jose S Pulido
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Alan D Marmorstein
    Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States
  • Footnotes
    Commercial Relationships   Jarel Gandhi, None; Fukutaro Mano, None; Stephen LoBue, None; Timothy Olsen, None; Raymond Iezzi, None; Jose Pulido, None; Alan Marmorstein, None
  • Footnotes
    Support  Gordon and Llura Gund Fund for Career Development in Retinal Degenerative Disease Research, the Alfred A. Iversen Family Foundation and PMT Corp., the VitreoRetinal Surgery Foundation Research Fellowship, a gift to the Mayo Clinic Center for Regenerative Medicine from Mr. Gene Wood, and Mayo Clinic charitable funds
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3941. doi:
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    • Get Citation

      Jarel K Gandhi, Fukutaro Mano, Stephen LoBue, Timothy W Olsen, Raymond Iezzi, Jose S Pulido, Alan D Marmorstein; Surgical Placement and Degradation of Fibrin Scaffolds within the Subretinal Space of a Porcine Eye. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3941.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have recently developed a biodegradable scaffold composed of human fibrin for use in RPE transplantation. In this study we developed a surgical procedure for placement of fibrin scaffolds in the subretinal space and tested the degradation kinetics and safety profile of fibrin scaffolds using an in vivo porcine subretinal implantation model.

Methods : Fibrin scaffolds were cast by mixing human fibrinogen (40mg/mL) and thrombin (50U/mL) solutions in a custom mold. Once polymerized, scaffolds of 1.5mm x 5.0mm x 0.2mm were obtained using a punch and stained with trypan blue for visualization. Scaffolds were placed in the subretinal space of one eye of a domestic pig (n >8) following partial vitrectomy and creation of a retinal bleb, using a novel inserter tool. Scaffold degradation was assessed postoperatively by fundus exam, OCT, and postmortem histology for up to 12 weeks.

Results : Immediately following implantation, the scaffold was visible under the retina. After 3 days, the retina appeared flat except at the implantation site, in which the elevation coincided with the thickness of the scaffold. Histology at this early time point confirmed the gel was placed successfully within the subretinal space without damage to the surrounding tissue. At 1 and 2 weeks, OCT images demonstrate scaffold degradation. By the 8th week, the scaffold appeared to have degraded in fundus exams and by OCT (n=4). Histologically, at the implantation site, the retina was reattached and morphologically we could not detect damage to the underlying RPE or the overlying neurosensory retina.

Conclusions : Fibrin scaffolds implanted in the subretinal space degrade within 8 weeks without any detectable adverse effects. Fibrin gels offer a bio-degradable scaffold for use in RPE transplantation, potentially improving upon current non-degradable scaffolds.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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