July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Digital Surgery: Manufacturing corneal Biopatches using a Piezoelectric Inkjet Printer
Author Affiliations & Notes
  • PRISCILA Cardoso CRISTOVAM
    OPHTHALMOLGY AND VISUAL SCIENCES, UNIFESP, São Paulo, Brazil
  • Juliana Lopes Hoehne
    OPHTHALMOLGY AND VISUAL SCIENCES, UNIFESP, São Paulo, Brazil
  • Regina Carlstron
    OPHTHALMOLGY AND VISUAL SCIENCES, UNIFESP, São Paulo, Brazil
  • silvia Itzcovici Abensur
    OPHTHALMOLGY AND VISUAL SCIENCES, UNIFESP, São Paulo, Brazil
  • Priscila Correia Antoneli
    OPHTHALMOLGY AND VISUAL SCIENCES, UNIFESP, São Paulo, Brazil
  • Paulo Schor
    OPHTHALMOLGY AND VISUAL SCIENCES, UNIFESP, São Paulo, Brazil
  • Footnotes
    Commercial Relationships   PRISCILA CRISTOVAM, None; Juliana Hoehne, None; Regina Carlstron, None; silvia Abensur, None; Priscila Antoneli, None; Paulo Schor, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 4134. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      PRISCILA Cardoso CRISTOVAM, Juliana Lopes Hoehne, Regina Carlstron, silvia Itzcovici Abensur, Priscila Correia Antoneli, Paulo Schor; Digital Surgery: Manufacturing corneal Biopatches using a Piezoelectric Inkjet Printer. Invest. Ophthalmol. Vis. Sci. 2019;60(9):4134.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Piezoelectric drop-on-demand inkjet 3d Bio-printing was developed to support various liquid substances, providing precise control over material deposition. The production of bioinks and printing protocols for this technology is under development and might be applyed to several laboratory and medical conditions, that require precise cell or othe biometaerial deposition. The purpose of this study was to test the feasibility of filling a digital image-based cornea wounds by bioinks.

Methods : Tissuejet 1000 (MicroFab Technologies, TX, USA) piezzoeletric dispenser was used to produce water based bioinks. Drop-on-demand bursts and continuous ejection were both used to achieve an optimal droplet regarding to size, drop velocity and trajectory.

- Bioink viability
Corneal epithelial cells (CEC) were obtained from corneoescleral rims, cultured in SHEM medium and expanded in vitro. CEC in suspension were poured through to the bioprint to a culture plate. Morphology and migration were evaluated by phase contrast microscopy and its viability analysed by MTT method.

- Biopatch printing protocol
Images of different types of human cornea lesions were obtained and digitally manipulated. A image compound of a white shape, with the same lesion shape, in a black background were created. This image was saved as monochrome bitmap to create the print script file to be applyed onto the corneal surface.

Results : Epithelial cell viability was determined by MTT analysis and cells integrity (OU CONFLEINCIA) were monitored using phase contrast microscopy for three days during its growth at 37 °C. Image manipulation of human corneal wound were possible to be obtained and digitally inverted to be inputed in the 3D bioprinter software. The script provided the cells and biomaterials to be accurately positioned in established patterns for printing corneal biopatches.

Conclusions : The development of printing protocols and the feasibility analysis of bioinks are tools that make viable the Piezoelectric Inkjet Printing technique for the manufacturing of corneal bio-patches. The protocol and bioink resulting from this work are being used for diverse customized precise microvolume biological applications by our group. This work is currently in progress and initial results have been promising.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×