Abstract
Purpose :
To investigate the role of the lipid mediator, resolvin D1 (RvD1), in corneal inflammation.
Methods :
C57BL/6 mouse corneas were abraded and treated with or without RvD1 followed by Pseudomonas aeruginosa stimulation. Development of corneal haze and edema were measured by photography and anterior optical coherence tomography. Neutrophil recruitment to the cornea and chemokine production were measured by immunohistochemistry and ELISA, respectively.
Results :
In resolvin treated group, corneal infiltrate and edema were significantly reduced and neutrophil recruitment was also significantly inhibited. Cytokine production such as IL-6 and CXCL1 was significantly reduced.
Conclusions :
RvD1 inhibits corneal inflammation induced by P. aeruginosa . These findings indicate that RvD1 as a potential anti-inflammatory therapy for patients with corneal inflammation and also, when given together with antibiotics, for bacterial keratitis.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.