July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Fortified Post-Thaw Amniotic Membrane with Platelet Lysate Potentiates Cultivation of Limbal Stem Cells
Author Affiliations & Notes
  • Mozhgan Rezaei Kanavi
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Samira Karami
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Sahar Balagholi
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Shaban Alizadeh
    Department of Hematology, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Hamid Ahmadieh
    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Rasul Dabbaghi
    Department of Hematology, Faculty of Medical sciences, Tarbiat Modares University, Tehran, Iran (the Islamic Republic of)
  • Footnotes
    Commercial Relationships   Mozhgan Rezaei Kanavi, None; Samira Karami, None; Sahar Balagholi, None; Shaban Alizadeh, None; Hamid Ahmadieh, None; Rasul Dabbaghi, None
  • Footnotes
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Investigative Ophthalmology & Visual Science July 2019, Vol.60, 4694. doi:
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      Mozhgan Rezaei Kanavi, Samira Karami, Sahar Balagholi, Shaban Alizadeh, Hamid Ahmadieh, Rasul Dabbaghi; Fortified Post-Thaw Amniotic Membrane with Platelet Lysate Potentiates Cultivation of Limbal Stem Cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):4694.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To survey the effects of fortified post-thaw human amniotic membranes (HAMs) by platelet lysate (PL) on proliferation and maintenance of overlying cultivated human limbal stem cells (hLSCs).

Methods : PL was obtained from 3 volunteers by adding thrombin solution in 1:5 ratio. HAMs were frozen and thawed followed by fortifying with PL. hLSCs derived from donated corneoscleral rings were cultured on post-thaw fortified HAMs and compared with those on post-thaw non-fortified HAMs (controls) in terms of cell viability and genotypic / phenotypic alterations.

Results : Cultivated hLSCs on post-thaw fortified HAMs, as compared to the controls, demonstrated a significant increase of viability on days 1 (P = 0.029) and 3 (P = 0.042), and a borderline increase after 1 week (P = 0.069). Gene expression profile showed a remarkable reduction of Tp63 on day 7 (P = 0.028) and a significant increase of NGFR on days 1, 3, and 7 (P = 0.028, P = 0.0001, and P = 0.0001, in that order). However, the expression of p63 protein was not significantly different between the cultivated LSCs on post-thaw fortified HAMs and the controls after 1 week (P = 0.18).

Conclusions : Post-thaw HAMs fortified by PL have a favorable effect on proliferation and differentiation of cultured hLSCs from stem cell identity to epithelial precursor stage, and can be advantageous over non-fortified HAMs for ocular surface wound healings and reepithelialization.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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