July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Neuroprotective effect of icariin in a rat model of anterior ischemic optic neuropathy.
Author Affiliations & Notes
  • CHIN-TE HUANG
    Department of Ophthalmology, Chung Shan Medical University Hospital, School of Medicine, Chung Shan Medical University, Taichung City, Taiwan
    Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan
  • Tushar Desai
    Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan
  • Felice Cheng
    Drug Delivery Lab, Biomedical Technology and Device Research Labs, Industrial Technology Research Institute, Hsinchu, Taiwan
  • Yao-Tseng Wen
    Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan
  • Rong-Kung Tsai
    Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan
    Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan
  • Footnotes
    Commercial Relationships   CHIN-TE HUANG, None; Tushar Desai, None; Felice Cheng, None; Yao-Tseng Wen, None; Rong-Kung Tsai, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 4857. doi:
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      CHIN-TE HUANG, Tushar Desai, Felice Cheng, Yao-Tseng Wen, Rong-Kung Tsai; Neuroprotective effect of icariin in a rat model of anterior ischemic optic neuropathy.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):4857.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Non-arteritic anterior ischemic optic neuropathy (NAION) is the most common acute optic neuropathy in patients over 50 years of age and most probably results from flow impairment to the anterior optic nerve, which generates optic nerve (ON) ischemia. We tested the neuroprotective effect of icariin treatment in the rat model of anterior ischemic optic neuropathy (rAION) to rescue retinal ganglion cells (RGCs).

Methods : Adult male Wistar rats weighing 150-180 g (7- to 8-weeks old) were used in this study. The ONHs of the rats were exposed to an argon laser immediately after an intravenous injection of the photosensitizing agent Rose Bengal. The other normal control eyes received a sham laser. Icariin (5 µM, 3µL) or phosphate-buffered saline (PBS control, 3 μL) was administered immediately by intravitreal injection before or after induction of rAION. We used optical coherence tomography to observe the change of the width of the optic head after icariin treatment to evaluate the presence of optic disc swelling. Visual function was assessed by flash visual evoked potentials (fVEP). RGC density was counted by retrograde labeling with FluoroGold.

Results : Optical coherence tomography of the optic head showed that the narrower width of optic head in the icariin-treated groups, no matter in group treated prior induction of rAION (221 µm) or in group treated post induction (200 µm), than that in the PBS control group (283 µm). The fVEP analysis showed the higher amplitude of the P1-N2 wave in the icariin-treated groups of prior- and post-induction groups (42±8 μV and 39±13 μV) than the PBS-treated group (17±6 μV). Two weeks after the insult, the RGC densities in the prior- and post-induction of HEP extract-treated groups were significantly higher (1666.72±250.9/mm2 and 1743.17±121.81/mm2) than that of the PBS-treated group (800.14±320.22/mm2).

Conclusions : The expected findings provide much crucial information about neuroprotective effect of icariin in response to ischemic stress in the rAION model.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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