Abstract
Purpose :
Staphylococcal nuclease and tudor domain containing 1 (SND1) is a multifunctional protein implicated in a variety of cellular processes. Here, we investigate the role of SND1 in the DNA damage response of cone photoreceptor cells and analyzed the mechanism responsible for the effects.
Methods :
Knocking down SND1 in cell line 661W, and cell proliferation, colony formation assays, flow cytometry, cell cycle analysis, comet assay and Immunofluorescence were evaluated after receiving X-ray irradiation. And associated pathways were detected by Western Blot.
Results :
Down-regulating SND1 in mouse-derived photoreceptor cell line 661W markedly inhibited cell proliferation and increased apoptosis after IR treatment. After DNA damage, SND1 activates the ATM signaling pathway to initiate DNA repair. Defects in SND1 are associated with a missing response to the DNA damage signal to cell cycle checkpoints or DNA repair.
Conclusions :
our study provides evidence that SND1 is a novel regulator of the DNA damage response, and may provide new insights into potential therapeutic targets in retinal damage caused by IR.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.