July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Characterization of human iPSC-derived corneal endothelial cells
Author Affiliations & Notes
  • Jiagang Zhao
    Shiley Eye Center, University of California San Diego, La Jolla, California, United States
  • Natalie A Afshari
    Shiley Eye Center, University of California San Diego, La Jolla, California, United States
  • Footnotes
    Commercial Relationships   Jiagang Zhao, None; Natalie Afshari, None
  • Footnotes
    Support  NIH Grants EY028983 and EY029166
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5085. doi:
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      Jiagang Zhao, Natalie A Afshari; Characterization of human iPSC-derived corneal endothelial cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5085.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : To probe whether human induced pluripotent stem cell-derived corneal endothelial cells (hiPSC-derived CEnCs) could be used as biologically equivalent of human primary cells.

Methods : The expression of a panel of CEnC specific markers are examined by both quantitative PCR and immunocytochemistry analyses. Cell proliferation, maturation and ability to form a corneal endothelium-like monolayer in culture have been studied.

Results : The iPSC-derived CEnCs remain proliferative and retain typical polygonal shape while forming a monolayer even after 10 passages in culture. The inhibition of cell signaling pathway that reduced YAP activity was found to dampen the cell growth and promote maturation of hiPSC-derived CEnCs in culture. The changes in the expression and distribution of corneal barrier, pump and scaffold proteins have been observed. The preference of cell growth and forming a monolayer on different substrates have also been investigated.

Conclusions : Our work demonstrates the potential of hiPSC-derived CEnCs, which can be generated consistently and unlimitedly in a laboratory, to serve as a useful biosimilar cell model for mechanistic and translational studies on corneal endothelial dystrophy.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.


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