July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
FGF9 regulates endothelial-to-mesenchymal transition of corneal endothelial cells
Author Affiliations & Notes
  • JINGWEN YU
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Shangkun Ou
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Huimin Sun
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Han Wu
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Yuli Guo
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Minjie Zhang
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Zuguo Liu
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Wei Li
    Eye Institute of Xiamen University, Xiamen, China
    Medical College of Xiamen University, Xiamen, China
  • Footnotes
    Commercial Relationships   JINGWEN YU, None; Shangkun Ou, None; Huimin Sun, None; Han Wu, None; Yuli Guo, None; Minjie Zhang, None; Zuguo Liu, None; Wei Li, None
  • Footnotes
    Support  2018YFA0107301
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5086. doi:https://doi.org/
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    • Get Citation

      JINGWEN YU, Shangkun Ou, Huimin Sun, Han Wu, Yuli Guo, Minjie Zhang, Zuguo Liu, Wei Li; FGF9 regulates endothelial-to-mesenchymal transition of corneal endothelial cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5086. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Corneal endothelial cells (CECs) can undergo endothelial-to-mesenchymal transition (EndMT) after injuries or during ex vivo culture. Fibroblast growth factor 9 (FGF9) is reported to reverse the epithelial-to-mesenchymal transition in prostate cancer cells and accelerate wound healing. Our current study aims to investigate the expression and function of FGF9 on CECs.

Methods : The expressions of FGF9 and its receptor FGFR3 on CECs of human and rabbit were detected by immunohistochemistry and PCR. Slit-lamp microscope observation was performed on day 0, day 4 and day 7 after corneal endothelial injuries. Endothelial cells were harvested at different time points and the gene expressions of N-cadherin, ZO-1, Na+-K+ -ATPase, α-SMA, FGF9, and FGFR3 were detected by qRT-PCR. Rabbit CECs were cultured in SHEM for 4 passages with or without exogenous FGF9. CCK8 and immunofluorescence staining of N-cadherin, ZO-1, Na+-K+ -ATPase, α-SMA, FGF9, FGFR3, and Ki67 were performed on FGF9-treated CECs.

Results : FGF9 and FGFR3 were expressed on both human and rabbit CECs. During rabbit corneal endothelium wound healing process and ex vivo culture, α-SMA was upregulated, while FGF9, FGFR3, N-cadherin, ZO-1, and Na+-K+ -ATPase were downregulated. Under the treatment of exogenous FGF9, α-SMA was down-regulated while FGF9, FGFR3, N-cadherin, ZO-1, and Na+-K+ -ATPase were upregulated in cultured CECs. Exogenous FGF9 had no effects on cell viability or proliferation.

Conclusions : FGF9 and its receptor FGFR3 are expressed on CECs. FGF9-FGFR3 signaling pathway is involved in the process of EndMT and phenotype transition of CECs.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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