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Hochul Yi, Young Joo Shin, Jin Sun Hwang, Tae-Young Chung; Effect of SOX2 Repression on Corneal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5110.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the effect of SOX2 repression on human corneal endothelial cells (HCECs).
Cultured HCECs were transfected by siRNA for SOX2. Cell viability and cell proliferation rate were measured. Cell proliferation-associated protein expression was evaluated by western blotting and RT-PCR. Cell shape and WNT signaling was assessed.
SOX2 expression was reduced 80% by siRNA for SOX2. Cell viability and proliferation rate was reduced. CDK and cyclin D1 expression reduced and CDKN2A expression increased. SIRT1 and ATP5B expression were reduced and pAMPK expression was elevated. Cell shape became elongated by siRNA for SOX2. WNT3A and β-catenin expression increased by siRNA for SOX2.
SOX2 repression may disrupt the normal wound healing process of HCECs. SOX2 may be a molecular target for corneal regeneration.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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