July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
A Comparative study of Cytotoxicities and Anti-allergic Effects by Topical Ocular Dual-action Anti-allergic Agents
Author Affiliations & Notes
  • Jong-Soo Lee
    Ophthalmology, Pusan National University College of medicine, Pusan, Korea (the Republic of)
    Medical Research Institute, Pusan National University Hospital, Pusan, Korea (the Republic of)
  • Ji-Eun Lee
    Ophthalmology, Pusan National University College of medicine, Pusan, Korea (the Republic of)
    Ophthalmology, Pusan National University Yangsan Hospital, Yangsan, Korea (the Republic of)
  • Seung il Kim
    Ophthalmology, Pusan National University College of medicine, Pusan, Korea (the Republic of)
    Ophthalmology, Pusan National University Yangsan Hospital, Yangsan, Korea (the Republic of)
  • Jong Heun Lee
    Ophthalmology, Pusan National University College of medicine, Pusan, Korea (the Republic of)
    Ophthalmology, Pusan National University Yangsan Hospital, Yangsan, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Jong-Soo Lee, None; Ji-Eun Lee, None; Seung il Kim, None; Jong Heun Lee, None
  • Footnotes
    Support  No
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5118. doi:
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      Jong-Soo Lee, Ji-Eun Lee, Seung il Kim, Jong Heun Lee; A Comparative study of Cytotoxicities and Anti-allergic Effects by Topical Ocular Dual-action Anti-allergic Agents. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5118.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The aim of this study was to investigate the cytotoxicities and anti-allergic effect of the topical ocular dual-action anti-allergic agents (alcaftadine 0.25%, bepotastine besilate 1.5%, and olopatadine HCL 0.1%) on cultured human corneal epithelial cells (HCECs) and conjunctival epithelial cells.

Methods : A methylthiazolyltetrazolium(MTT)-based calorimetric assay was used to assess cytotoxicities using HCECs at concentrations of 10, 20 or 30% for exposure durations of 30 min, 1h, 2h, 12h or 24h. Cellular morphologies were evaluated by inverted phase-contrast and electron microscopy and wound scratch test. Real-time PCR was used to quantify anti-allergic effects on cultured human conjunctival cells treated with Aspergillus antigen.

Results : Cell viabilities decreased in time- and concentration-dependent manners. Cells showed microvilli loss, cytoplasmic vacuoles, and nuclear condensation when exposed to anti-allergic agents; alcaftadine was found to be least cytotoxic. Alcaftadine treated HCECs monolayers exhibited the best wound healing followed by bepotastine and olopatadine (p<0.001). All agents significantly reduced the gene expressions of allergic cytokines (IL-5, IL-25, eotaxin, thymus and activation-regulated chemokine, thymic stromal lymphopoietin) and alcaftadine had the greatest effect (p<0.001 in all cases).

Conclusions : Alcaftadine seems to have less side effects and better therapeutic effects than the other two anti-allergic agents tested. It may be more beneficial to use less toxic agents for patients with ocular surface risk factors or presumed symptoms of toxicity.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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