Abstract
Purpose :
Zebrafish is an ideal biological model for studying human diseases, because of many similarities with the human genome. The process of pathological angiogenesis is responsible for lots of medical complications in human body like wet age-related macular degeneration (wAMD) and proliferative diabetic retinopathy (PDR). The aim of the study was to present the influence of Dopamine’s agonist on the angiogenesis of Zebrafish larvae.
Methods :
3 substances were used: bromocriptine, pergolide, cabergoline in concentration of o 2,5 μmol. Process of angiogenesis was observed for 5 days in larvae of genetically modified ZebrafishFli with labelled, luminescent blood vessels. Photo documentation was taken under the fluorescent microscope Stereo discover v8, fluorescence 200c hxp Carl Zeiss Microscopy GmbH. The level of VEGF and VEGFR2 gene expression was measured using rt-PCR method.
Results :
Tested substances inhibited process of angiogenesis in Zebrafish larvae. The VEGF and VEGFR2 expression was lower in the presence of dopamine agonists than in a control
Conclusions :
Inhibition of angiogenesis by dopamine’s agonist may be new therapeutic option in treatment of diseases like wet age-related macular degeneration and proliferative diabetic retinopathy.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.