July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Dopamine agonists- a new way to inhibit pathological angiogenesis in zebrafish model
Author Affiliations & Notes
  • Swiech- Zubilewicz Anna
    Department of Retina and Vitreus Surgery, Medical University of Lublin, Poland, Lublin, Poland
  • Katarzyna Danieluk
    Department of Retina and Vitreus Surgery, Medical University of Lublin, Poland, Lublin, Poland
    2. Centre of Experimental Medicine, Medical University of Lublin, Poland, Lublin, Poland
  • Joanna Dolar-Szczasny
    Department of Retina and Vitreus Surgery, Medical University of Lublin, Poland, Lublin, Poland
  • Maciej Oseka
    Oftalabs, Warsaw, Poland
  • Jerzy Mackiewicz
    Department of Retina and Vitreus Surgery, Medical University of Lublin, Poland, Lublin, Poland
  • Footnotes
    Commercial Relationships   Swiech- Zubilewicz Anna, None; Katarzyna Danieluk, None; Joanna Dolar-Szczasny, None; Maciej Oseka, Oftalabs (P); Jerzy Mackiewicz, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5402. doi:
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      Swiech- Zubilewicz Anna, Katarzyna Danieluk, Joanna Dolar-Szczasny, Maciej Oseka, Jerzy Mackiewicz; Dopamine agonists- a new way to inhibit pathological angiogenesis in zebrafish model. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5402.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Zebrafish is an ideal biological model for studying human diseases, because of many similarities with the human genome. The process of pathological angiogenesis is responsible for lots of medical complications in human body like wet age-related macular degeneration (wAMD) and proliferative diabetic retinopathy (PDR). The aim of the study was to present the influence of Dopamine’s agonist on the angiogenesis of Zebrafish larvae.

Methods : 3 substances were used: bromocriptine, pergolide, cabergoline in concentration of o 2,5 μmol. Process of angiogenesis was observed for 5 days in larvae of genetically modified ZebrafishFli with labelled, luminescent blood vessels. Photo documentation was taken under the fluorescent microscope Stereo discover v8, fluorescence 200c hxp Carl Zeiss Microscopy GmbH. The level of VEGF and VEGFR2 gene expression was measured using rt-PCR method.

Results : Tested substances inhibited process of angiogenesis in Zebrafish larvae. The VEGF and VEGFR2 expression was lower in the presence of dopamine agonists than in a control

Conclusions : Inhibition of angiogenesis by dopamine’s agonist may be new therapeutic option in treatment of diseases like wet age-related macular degeneration and proliferative diabetic retinopathy.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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