July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Evaluation of oxidative stress profiles of the serum and aqueous humor in patients with open-angle glaucoma
Author Affiliations & Notes
  • YASUYUKI TAKAI
    Ophthalmology, Shimane University Faculty of Medicine, Enya 89-1, Izumo, Japan
  • KAZUNOBU SUGIHARA
    Ophthalmology, Shimane University Faculty of Medicine, Enya 89-1, Izumo, Japan
  • Sachiko Kaidzu
    Ophthalmology, Shimane University Faculty of Medicine, Enya 89-1, Izumo, Japan
  • Masaki Tanito
    Ophthalmology, Shimane University Faculty of Medicine, Enya 89-1, Izumo, Japan
  • Footnotes
    Commercial Relationships   YASUYUKI TAKAI, None; KAZUNOBU SUGIHARA, None; Sachiko Kaidzu, None; Masaki Tanito, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5644. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      YASUYUKI TAKAI, KAZUNOBU SUGIHARA, Sachiko Kaidzu, Masaki Tanito; Evaluation of oxidative stress profiles of the serum and aqueous humor in patients with open-angle glaucoma. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5644.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose :
The involvement of local and systemic oxidative stresses in intraocular pressure (IOP) elevation and optic nerve damage has been hypothesized in the pathogenesis of glaucoma. To test this, we measured the systemic levels of prooxidants and antioxidants, and the activities of antioxidant enzymes in the aqueous humor.

Methods :
Blood and aqueous humor samples were obtained from Japanese subjects with primary open-angle glaucoma (PG) (n=20), exfoliation glaucoma (EXG) (n=20) and nonglaucomatous controls (n=20). Serum levels of lipid peroxides, ferric-reducing activity, and thiol antioxidant activity were measured by diacron reactive oxygen metabolites (dROM), biological antioxidant potential (BAP), and sulfhydryl (SH) tests, respectively, using a free radical analyzer. The activities of cytosolic (SOD1) and mitochondrial (SOD2) forms of superoxide dismutase isoform in aqueous humor were measured using enzyme-linked immunosorbent assay technique.

Results : Compared to control subjects, the BAP values were significantly lower (P<0.05) in PG and EXG subjects. Compared to control eyes, SOD1 and SOD2 concentration were significantly higher (P<0.05) in PG and EXG eyes. The BAP values were negatively correlated with SOD1 and SOD2 concentrations, IOP and the number of antiglaucoma medications.

Conclusions : Lower systemic antioxidant capacity accompanies up-regulation of higher local antioxidant enzymes, suggesting an increased oxidative stress in eyes with open-angle glaucoma. Determination of systemic BAP values may help in predicting the redox status in aqueous humor and IOP.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×