July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Identification of the Aqueous Humor Proteins correlated with Intraocular Pressure in Primary Open Angle Glaucoma Patients.
Author Affiliations & Notes
  • Sai Karthik Kodeboyina
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • TaeJin Lee
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • Shruti Sharma
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
    Department of Ophthalmology, Augusta University, Augusta, Georgia, United States
  • Wenbo Zhi
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
  • Kathryn E Bollinger
    Department of Ophthalmology, Augusta University, Augusta, Georgia, United States
    Department of Cellular Biology and Anatomy, Augusta University, Augusta, Georgia, United States
  • Lane Ulrich
    Department of Ophthalmology, Augusta University, Augusta, Georgia, United States
  • Ashok Sharma
    Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, Georgia, United States
    Department of Population Health Sciences, Augusta University, Augusta, Georgia, United States
  • Footnotes
    Commercial Relationships   Sai Karthik Kodeboyina, None; TaeJin Lee, None; Shruti Sharma, None; Wenbo Zhi, None; Kathryn Bollinger, None; Lane Ulrich, None; Ashok Sharma, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5652. doi:
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      Sai Karthik Kodeboyina, TaeJin Lee, Shruti Sharma, Wenbo Zhi, Kathryn E Bollinger, Lane Ulrich, Ashok Sharma; Identification of the Aqueous Humor Proteins correlated with Intraocular Pressure in Primary Open Angle Glaucoma Patients.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5652.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Intraocular pressure (IOP) remains one of the greatest risk factors for Primary Open Angle Glaucoma (POAG). The relationship between IOP and the aqueous humor (AH) proteome in glaucoma is unknown. The purpose of this study was to discover the AH proteins correlated with IOP in POAG patients.

Methods : AH samples from 15 POAG patients were analyzed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Pearson correlation analyses were performed to discover the relationship between the AH protein levels and IOP values. Bioinformatics analyses were conducted to identify biological pathways, protein interaction networks and the cellular functions of the identified proteins.

Results : A total of 43 AH proteins were significantly correlated (p-value<0.05) with IOP in POAG patients. Thirty-five proteins were positively correlated whereas 8 proteins were negatively correlated with IOP. The top 10 proteins with a significant positive correlation are: ITM2B (ρ=0.841), CLSTN1 (ρ=0.837), APOE (ρ=0.822), SERPINF1 (ρ=0.764), CLU (ρ=0.737), APLP1 (ρ=0.708), APLP2 (ρ=0.685), LGALS3BP (ρ=0.678), HEL-S-130P (ρ=0.675), NPC2 (ρ=0.663). The top 5 proteins negatively correlated proteins with IOP are: HSD17B10 (ρ=-0.634), TRBV24-1 (ρ=-0.622), CD22 (ρ=-0.621), IDH3A (ρ=-0.620), and ZNF251 (ρ=-0.565). Bioinformatics analyses revealed many significantly enriched biological functions among the positively correlated proteins including, lipid and cholesterol metabolism, cell death and survival, hereditary eye disease and neovascularization of eye. Biological process enriched among the negatively correlated proteins include, immune response, carbohydrate and lipid metabolism and regulation of transcription.

Conclusions : We identified several proteins in the AH of POAG patients correlated with IOP. We also highlighted the functional relevance of these proteins. Particularly, lipid metabolism was significantly enriched among the positively correlated proteins, reinforcing the idea that dysregulated lipid metabolism is involved in the pathogenesis of POAG. The association of proteins levels with a major risk factor for glaucoma highlights their plausible functional relationship in PAOG pathogenesis.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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