July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
AQUEOUS HUMOR β42-AMYLOID AND TOTAL PROTEINS CONCENTRATION IN GLAUCOMATOUS PATIENTS
Author Affiliations & Notes
  • Francesca Cappelli
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Maria Marenco
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Valeria Testa
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Alessandro Masala
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Francesca Caudano
    Department of Experimental Medicine, Section of General Pathology, School of Medical and Pharmaceutical Sciences, University of Genova, 16132 Genova, Italy, Italy
  • Daniele Sindaco
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Angelo Macrì
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Carlo Enrico Traverso
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Roberta Ricciarelli
    Department of Experimental Medicine, Section of General Pathology, School of Medical and Pharmaceutical Sciences, University of Genova, 16132 Genova, Italy, Italy
  • Michele M Iester
    Eye Clinic of Genoa, Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16132 Genova, Italy., Italy
  • Footnotes
    Commercial Relationships   Francesca Cappelli, None; Maria Marenco, None; Valeria Testa, None; Alessandro Masala, None; Francesca Caudano, None; Daniele Sindaco, None; Angelo Macrì, None; Carlo Traverso, None; Roberta Ricciarelli, None; Michele Iester, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5656. doi:
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      Francesca Cappelli, Maria Marenco, Valeria Testa, Alessandro Masala, Francesca Caudano, Daniele Sindaco, Angelo Macrì, Carlo Enrico Traverso, Roberta Ricciarelli, Michele M Iester; AQUEOUS HUMOR β42-AMYLOID AND TOTAL PROTEINS CONCENTRATION IN GLAUCOMATOUS PATIENTS. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5656.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Glaucoma shares some features with Alzheimer disease and other neurodegenerative disorders. We tested the association between glaucoma and total proteins (TP) and/or A-β42 concentration in the aqueous humor in a prospective clinical study.

Methods : Eighty-four patients were consecutively enrolled, 37 males and 47 females with a median age of 77 years. 86 eyes were examined, 34 were affected by glaucoma and 52 were healthy. Ophthalmological examination, biometry with Nidek Al-Scan and Lenstar Haag-Streit LS900 (axial length, anterior chamber depth, central corneal thickness were recorded), intraocular pressure (IOP) measurements with Goldman applanation tonometry, fundus examination, ganglion cells complex (mean thickness) and/or Retinal Nerve Fiber Layer (mean thickness) analysis with Optovue RTVue RT-100 OCT were performed unless unfeasible due to media opacities. Anti-glaucomatous pharmacological therapy was recorded. All patients were candidates for cataract surgery and were grouped according to the presence of glaucoma. During the procedure, an aqueous humor sample was collected and later processed with Enzyme-Linked Immunosorbent Assay (ELISA) method to detect TP and Aβ42 concentrations.

Results : A significant difference (p<0.05) was found for TP between healthy and glaucomatous subjects (3.38±1.03 mg/ml and 4.05±1.5 pM (mean± SD) respectively). A significant correlation was found between TP and A-β42 (r.0.45, p=0.002), pre-IOP (r.45, p<0.002) and age(r=-0.29, p<0.05). No difference was found between A-β42 and all the considered parameters between glaucomatous and healthy subjects.

Conclusions : Despite the lack of correlation between A-β42 aqueous humor concentration and the other parameters in glaucomatous patients, a significant correlation was found for TP concentration. This result outlined a possible role of aqueous humor proteins in glaucomatous disease pathogenesis.
Further studies on a large sample are thus warranted.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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