July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Effects of North American Ginseng Extracts on Lens Health and Plasma in Streptozotocin Diabetic Rats Using Early and Late Treatment
Author Affiliations & Notes
  • Tomasz M Dzialoszynski
    Faculty of Health Sciences, Western University, London, Ontario, Canada
  • Victoria Jaremek
    Anatomy & Cell Biology, Western University, London, Ontario, Canada
  • Earl Noble
    Faculty of Health Sciences, Western University, London, Ontario, Canada
  • Kenneth P Mitton
    Eye Research Institute, Pediatric Retinal Research Lab, Oakland University, Rochester, Michigan, United States
  • Morris Karmazyn
    Physiology & Pharmacology, Western University, London, Ontario, Canada
  • John R. Trevithick
    Faculty of Health Sciences, Western University, London, Ontario, Canada
  • Footnotes
    Commercial Relationships   Tomasz Dzialoszynski, None; Victoria Jaremek, None; Earl Noble, None; Kenneth Mitton, None; Morris Karmazyn, None; John Trevithick, None
  • Footnotes
    Support  Ministry of Research & Innovation in Ontario, Ontario Innovation Research Consortium, Western Health Sciences
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5688. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Tomasz M Dzialoszynski, Victoria Jaremek, Earl Noble, Kenneth P Mitton, Morris Karmazyn, John R. Trevithick; Effects of North American Ginseng Extracts on Lens Health and Plasma in Streptozotocin Diabetic Rats Using Early and Late Treatment. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5688.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the effects of aqueous ginsenoside extracts from North American ginseng on blood lipid profile, lens antioxidant status and lens heat shock proteins in Streptozotocin (STZ) type 1 diabetic rats.

Methods : Sprague-Dawley rats were divided into five groups of 16 rats each: 1. 12-weeks normal, 2. 12-weeks diabetic, 3. 12-weeks diabetic ginseng-treated, , 4. 6-weeks diabetic, followed by 6-weeks ginseng treatment (6WG), 5. 6-weeks diabetic. Animals were made diabetic (blood glucose ≥18 mM) by injecting (IP) with 20 mg/kg STZ for 4 consecutive days. North American ginseng aqueous extract (250 mg/kg in drinking water) was administered daily to 6-week diabetic rats. The general pathology of diabetes was assessed using body weight, blood lipid profile, HbA1c level, cataract formation, glutathione (GSH), plasma malondialdehyde (MDA) and total antioxidant level in various tissues.

Results : Lens GSH content was decreased in the diabetic-control group. 6-week ginseng treatment (6WG) significantly increased GSH concentration relative to diabetic group. Plasma glucose, triglycerides, cholesterol/HDL and HbA1c were elevated, and animal weight was decreased in diabetic rats. Ginseng treatment generally reduced triglycerides in diabetic rats. Both early and late treatment with ginseng decreased MDA, triglycerides and cholesterol in diabetic rats. Total antioxidant level in diabetic lenses and plasma decreased compared to normal rats. Cataract grade, measured by ScantoxR, was not significantly changed by treatment. Focusing ability measured by NIH contrast “ImageJ” although decreased in diabetes, was lower if only treated with 6 weeks diabetic ginseng.

Conclusions : Dietary ginsenoside extracts from North American ginseng started immediately or even delayed for 6-weeks has beneficial effects on preserving more normal GSH levels in the lens and triglyceride levels in plasma in a type 1 diabetic rat, even without insulin treatment.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×