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Zhaohua Yu, Per Söderberg; In vivo dose-response effect of UVR-300nm on density of lens epithelial cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5699.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the effect of different doses of UVR on the distribution of lens epithelial cells (LECs).
Altogether, 40 Sprague Dawley rats were unilaterally exposed in vivo to 1, 3, 6 and 8 kJ/m2 UVR-300 nm for 15 minutes. One week after UVR exposure, exposed and contralateral non-exposed lenses were removed. One midsagittal section from each lens was stained with DAPI for fluorescence microscopy in order to determine the spatial distribution of LECs.
The LECs density difference between exposed and contralateral non-exposed eyes at exposure dose of 1, 3, 6 and 8 kJ/m2 was estimated as a CI(95%), -0.1±2.1, 0.1±3.0, -4.1±1.4 and -2.9±2.7 Cell × μm-1 ×10-2, respectively. Data was fit to a linear model considering the initial density difference at 0. The inclination coefficient was estimated as CI(95%), -0.4 ± 0.1 Cell × μm × kJ-1 ×1010.
UVR-300 nm induces significant LECs density decrease at exposure dose ≥ 6 kJ/m2. With fitting to a linear model the evolution of dose- cell density dependence can be determined.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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