July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
In vivo dose-response effect of UVR-300nm on density of lens epithelial cells
Author Affiliations & Notes
  • Zhaohua Yu
    Neuroscience/Ophthalmology, Uppsala university, Uppsala, Sweden
  • Per Söderberg
    Neuroscience/Ophthalmology, Uppsala university, Uppsala, Sweden
  • Footnotes
    Commercial Relationships   Zhaohua Yu, None; Per Söderberg, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5699. doi:
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      Zhaohua Yu, Per Söderberg; In vivo dose-response effect of UVR-300nm on density of lens epithelial cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5699.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine the effect of different doses of UVR on the distribution of lens epithelial cells (LECs).

Methods : Altogether, 40 Sprague Dawley rats were unilaterally exposed in vivo to 1, 3, 6 and 8 kJ/m2 UVR-300 nm for 15 minutes. One week after UVR exposure, exposed and contralateral non-exposed lenses were removed. One midsagittal section from each lens was stained with DAPI for fluorescence microscopy in order to determine the spatial distribution of LECs.

Results : The LECs density difference between exposed and contralateral non-exposed eyes at exposure dose of 1, 3, 6 and 8 kJ/m2 was estimated as a CI(95%), -0.1±2.1, 0.1±3.0, -4.1±1.4 and -2.9±2.7 Cell × μm-1 ×10-2, respectively. Data was fit to a linear model considering the initial density difference at 0. The inclination coefficient was estimated as CI(95%), -0.4 ± 0.1 Cell × μm × kJ-1 ×1010.

Conclusions : UVR-300 nm induces significant LECs density decrease at exposure dose ≥ 6 kJ/m2. With fitting to a linear model the evolution of dose- cell density dependence can be determined.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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