Abstract
Purpose :
To determine the effect of different doses of UVR on the distribution of lens epithelial cells (LECs).
Methods :
Altogether, 40 Sprague Dawley rats were unilaterally exposed in vivo to 1, 3, 6 and 8 kJ/m2 UVR-300 nm for 15 minutes. One week after UVR exposure, exposed and contralateral non-exposed lenses were removed. One midsagittal section from each lens was stained with DAPI for fluorescence microscopy in order to determine the spatial distribution of LECs.
Results :
The LECs density difference between exposed and contralateral non-exposed eyes at exposure dose of 1, 3, 6 and 8 kJ/m2 was estimated as a CI(95%), -0.1±2.1, 0.1±3.0, -4.1±1.4 and -2.9±2.7 Cell × μm-1 ×10-2, respectively. Data was fit to a linear model considering the initial density difference at 0. The inclination coefficient was estimated as CI(95%), -0.4 ± 0.1 Cell × μm × kJ-1 ×1010.
Conclusions :
UVR-300 nm induces significant LECs density decrease at exposure dose ≥ 6 kJ/m2. With fitting to a linear model the evolution of dose- cell density dependence can be determined.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.