July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Real-time evaluation of systemic-ocular microcirculation using laser speckle flowgraphy in white rabbits
Author Affiliations & Notes
  • Tetsuya Komatsu
    Opthalmology, Toho university, Ota city, Tokyo, Japan
  • Tomoaki Shiba
    Opthalmology, Toho university, Ota city, Tokyo, Japan
  • Yoshinobu Nagasawa
    Pharmacology, Toho university, Japan
  • Megumi Aimoto
    Pharmacology, Toho university, Japan
  • Kiyoshi Sakuma
    Pharmacology, Toho university, Japan
  • Tatsuo Chiba
    Pharmacology, Toho university, Japan
  • Xin Cao
    Pharmacology, Toho university, Japan
  • Akira Takahara
    Pharmacology, Toho university, Japan
  • Tadashi Matsumoto
    Opthalmology, Toho university, Ota city, Tokyo, Japan
  • Yuichi Hori
    Opthalmology, Toho university, Ota city, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Tetsuya Komatsu, None; Tomoaki Shiba, None; Yoshinobu Nagasawa, None; Megumi Aimoto, None; Kiyoshi Sakuma, None; Tatsuo Chiba, None; Xin Cao, None; Akira Takahara, None; Tadashi Matsumoto, None; Yuichi Hori, HOYA (F), KOWA (F), Menicon (F), Senju (F)
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5730. doi:
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      Tetsuya Komatsu, Tomoaki Shiba, Yoshinobu Nagasawa, Megumi Aimoto, Kiyoshi Sakuma, Tatsuo Chiba, Xin Cao, Akira Takahara, Tadashi Matsumoto, Yuichi Hori; Real-time evaluation of systemic-ocular microcirculation using laser speckle flowgraphy in white rabbits. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5730.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To establish systemic-ocular microcirculation using laser speckle flowgraphy (LSFG) in white rabbits and investigate the circulatory response to a systemic adrenaline load.

Methods : We used six normal adult male New Zealand white rabbits (16 weeks of age, 2.8–3.3 kg). After intramuscular administration of xylazine (5 mg/kg) and ketamine (35 mg/kg), the rabbits were intubated and administered vaporized isoflurane (1.5-2.0%, room air 2 L/min). Surgical indwelling needles were inserted into the right and left auricular veins; these were used for infusion and drug administration. Heparinized arterial cannulas were placed in the right brachial and right rectal arteries for measurement of blood pressure while the blood flow rates of the right femoral artery and the left common carotid artery were measured using laser Doppler to determine blood flow volume in each artery. Mean blur rate (MBR) of the retinal vessels and choroid area were measured using LSFG-rabbit™ (Softcare, Fukuoka, Japan), developed for animals. Continuous intravenous adrenaline (0.2 mL/kg /min; 100-1000 ng/kg) was administered for 10 minutes, and changes in each index were observed for 30 minutes after the start of administration.

Results : The MBR increased in the retinal vessels and choroid area in a dose-dependent manner with the adrenaline load. The Δ mean blood pressure showed a significant correlation (R = 0.37 to 0.77) with each area of ΔMBR.
The Δ carotid blood flow volume showed a significant negative correlation with Δ retinal blood vessel MBR at 100-300 ng/kg dose and the Δ femoral arterial blood flow volume showed a significant positive correlation with Δ choroid area of MBR at each dose.

Conclusions : We successfully demonstraited a real-time evaluation system for systemic-ocular microcirculation using LSFG in white rabbits.
A systemic adrenaline load caused an increase in MBR, associated with elevation in blood pressure.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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