July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Determination of absolute diastolic erythrocyte velocities in peripapillary arterioles and venuoles of human subjects using erythrocyte mediated angiography
Author Affiliations & Notes
  • Victoria Chen
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Breanna Tracey
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Christopher Le
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Corinne Renner
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Jiaqi Li
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Lakyn Mayo
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Joby Tsai
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Michael Ou
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Sachin Kalarn
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Lily Im
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Mona Kaleem
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Osamah Saeedi
    Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Victoria Chen, None; Breanna Tracey, None; Christopher Le, None; Corinne Renner, None; Jiaqi Li, None; Lakyn Mayo, None; Joby Tsai, None; Michael Ou, None; Sachin Kalarn, None; Lily Im, None; Mona Kaleem, None; Osamah Saeedi, Heidelberg Engineering (F), Vasoptic Medical Inc (F)
  • Footnotes
    Support  NIH Grant K23 EY025014
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5738. doi:
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      Victoria Chen, Breanna Tracey, Christopher Le, Corinne Renner, Jiaqi Li, Lakyn Mayo, Joby Tsai, Michael Ou, Sachin Kalarn, Lily Im, Mona Kaleem, Osamah Saeedi; Determination of absolute diastolic erythrocyte velocities in peripapillary arterioles and venuoles of human subjects using erythrocyte mediated angiography. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5738.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Erythrocyte-mediated angiography (EMA) is a novel technique that permits direct visualization of indocyanine green (ICG)-labeled erythrocyte ghost cells in vivo. This allows for the measurement of individual erythrocyte velocities and observation of erythrocyte dynamics. We performed a cross-sectional human study to measure erythrocyte velocity in the peripapillary retinal microvasculature using EMA.

Methods : A Heidelberg Retinal Angiograph 2 (Heidelberg Engineering, Germany) was used to acquire 12-20 second angiograms of 17 participants undergoing EMA at 24.6 frames per second. After image registration with a semi-automated MATLAB script, a certified grader measured distances traveled by individual ICG labelled erythrocyte ghosts between frames in peripapillary non-branching vessels ranging from 30 to 65 microns in diameter. Distance traveled was measured in pixels and then converted to microns using patients’ corneal curvature from keratometry values and angiogram scaling from the Heidelberg imaging platform. Time was measured in frames and converted to seconds using the frame rate conversion (24.6 frames per second). Average diastolic velocity for a vessel was defined as the average of all tracked erythrocyte velocities at and below the 50th percentile in a single angiogram.

Results : 100 angiograms of 53 vessels from 17 individuals (8 females and 9 males) were analyzed. The average age of participants was 57.4 ± 5.9 years. The average diastolic venous velocity was 4.6 ± 1.0 mm/s. The average diastolic arterial velocity was 5.2 ± 1.8 mm/s. We observed a statistically significant difference between average diastolic venous and arterial velocities was observed (p=0.03).

Conclusions : Using erythrocyte mediated angiography, the average diastolic erythrocyte velocity in this sample was determined to be 4.6 ± 1.0 mm/s in human retinal venules and 5.2 ± 1.8 mm/s in arterioles. Further development and use of EMA may improve our understanding of ocular disease.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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