July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
A study of degradation controlling of the oligo-Tetra-PEG hydrogels applied for an artificial vitreous body.
Author Affiliations & Notes
  • Tomohiko Fujii
    Bioengineering Institute, R&D Div., NIDEK CO., LTD., Gamagori, Aichi, Japan
  • Yuko Shinohara
    Bioengineering Institute, R&D Div., NIDEK CO., LTD., Gamagori, Aichi, Japan
  • Masayoshi Nakatani
    Bioengineering Institute, R&D Div., NIDEK CO., LTD., Gamagori, Aichi, Japan
  • Sujin Hoshi
    Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Fumiki Okamoto
    Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Takamasa Sakai
    Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan
    Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan
  • Tetsuro Oshika
    Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Footnotes
    Commercial Relationships   Tomohiko Fujii, NIDEK CO., LTD. (E); Yuko Shinohara, NIDEK CO., LTD. (E); Masayoshi Nakatani, NIDEK CO., LTD. (E); Sujin Hoshi, NIDEK CO., LTD. (F), Tsukuba University (P); Fumiki Okamoto, Alconphama (F), Bayer (F), NIDEK CO., LTD. (F), Pfizer (F), Santen (F), Tsukuba University (P); Takamasa Sakai, NIDEK CO., LTD. (F), The University of Tokyo (P); Tetsuro Oshika, Alcon (F), Alcon (C), HOYA (F), HOYA (C), Jhonsonn & Jhonson Vision (F), Jhonsonn & Jhonson Vision (C), Kai (F), Kowa (F), Mitsubishi Tanabe (F), Novartis (F), Otsuka (F), Pfizer (F), Santen (F), Santen (C), Senju (F), Tomey (F), Topcon (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5793. doi:
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      Tomohiko Fujii, Yuko Shinohara, Masayoshi Nakatani, Sujin Hoshi, Fumiki Okamoto, Takamasa Sakai, Tetsuro Oshika; A study of degradation controlling of the oligo-Tetra-PEG hydrogels applied for an artificial vitreous body.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5793.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Development of novel biomaterials for implantation into the eyes is one of the important issues in vitreous surgery. Our team is developing the biocompatible and biodegradable oligo-Tetra-PEG hydrogels as an artificial vitreous body. The oligo-Tetra-PEG gel is formed rapidly via “critical-gel cluster”, has extremely low swelling pressure because of its ultralow polymeric content, and dissociates naturally after a certain period of time. In order to control the degradation period in eyes, we investigated the effect of the ratio of degradable cluster in the hydrogel on the degradation under in vitro accelerated condition.

Methods : Three types of 4arms-PEG reagents, which have respectively thiol (abbreviated to SH), maleimide (MA), and acrylate (ACR) at the terminal of their arms, were used for preparing non-degradable MAex-SH and hydrolyzable SHex-ACR clusters. 2 mL of 6 g/L hydrogel prepared from mixture of MAex-SH and SHex-ACR at the ratio of 1-R: R (R means the degradable SHex-ACR rate) was immerged in pH 10 phosphate buffer and a relation expression between R value and its degradation period was calculated by regression analysis. Moreover, the acceleration rate was calculated by compared with those immerged in DPBS, 35 °C.

Results : We tuned the ratio of degradable and non-degradable oligo-Tetra PEGs, and observed the longer gelation time (R=0.50, <88sec; R=0.58, 178 sec), and the lower equilibrium storage modulus (R=0.50, 18.6 Pa; R=0.58, 7.7 Pa) as increase of R value. Moreover, the mixture of clusters did not gelled above R=0.75, which was the critical point of gelation. The aqueous solution at pH 10 was selected so that only the ester bond was cleavage. At pH 10 buffer, the degradation rate was 50 times higher than that in DPBS. Based on the results, we estimated the equation predicting the dissociation period from R under accelerated condition. According to the equation and the acceleration rate, we found that the dissociation time of 90 days in DPBS, which is enough period for retinopexy, is achieved at R = 0.618.

Conclusions : We estimated the equation predicting the dissociation period from R under in vitro accelerated condition, which showed the dissociation time of 90 days in DPBS at R = 0.618. The period is considered to be enough for retinopexy.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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