July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
In vitro assessment of the ability of non-swelling polyethylene glycol based artificial vitreous hydrogel to maintain transparency in the presence of vitreous hemorrhage
Author Affiliations & Notes
  • Sujin Hoshi
    Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Japan
  • Fumiki Okamoto
    Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Japan
  • Tomoya Murakami
    Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Japan
  • Takamasa Sakai
    Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Japan
  • Masayoshi Nakatani
    Nidek Co., Ltd., Japan
  • Yuko Shinohara
    Nidek Co., Ltd., Japan
  • Tomohiko Fujii
    Nidek Co., Ltd., Japan
  • Tetsuro Oshika
    Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Japan
  • Footnotes
    Commercial Relationships   Sujin Hoshi, Nidek Co., Ltd. (P), Nidek Co., Ltd. (F); Fumiki Okamoto, Alcon Pharma (F), Nidek Co., Ltd. (P), Nidek Co., Ltd. (F); Tomoya Murakami, None; Takamasa Sakai, None; Masayoshi Nakatani, Nidek Co., Ltd. (E); Yuko Shinohara, Nidek Co., Ltd. (E); Tomohiko Fujii, Nidek Co., Ltd. (E); Tetsuro Oshika, Alcon Japan (F), Santen Pharmaceutical Co., Ltd. (F), TOMEY corp. (F)
  • Footnotes
    Support  AMED Grant Number JP17lm0203022
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5796. doi:
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      Sujin Hoshi, Fumiki Okamoto, Tomoya Murakami, Takamasa Sakai, Masayoshi Nakatani, Yuko Shinohara, Tomohiko Fujii, Tetsuro Oshika; In vitro assessment of the ability of non-swelling polyethylene glycol based artificial vitreous hydrogel to maintain transparency in the presence of vitreous hemorrhage. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5796.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Postoperative vitreous hemorrhage is a common, vision-impacting complication of vitrectomy. Intraocular tamponade with an artificial hydrogel at the end of surgery could possibly maintain transparency in the vitreous cavity, regardless of postoperative vitreous hemorrhage. This study was conducted to assess the ability of a non-swelling polyethylene glycol based artificial vitreous hydrogel to maintain transparency in the presence of vitreous hemorrhage in vitro.

Methods : One milliliter of diluted blood at concentrations of 0.1, 0.25, 0.5, and 1.25% was added to 1 mL each of polymerized hydrogel filled in plastic cuvettes (Gel + Blood group). Two milliliters of diluted blood at the same concentrations were prepared as controls (Blood group). Samples were left on a shaking apparatus. Spectral transmission curves of both groups were measured using a spectrophotometer before, and 1, 2, 5, 7, 14, and 28 days after, the start of the experiment. Percentage of transmittance in the visible light spectrum (400 - 700 nm) at each time point was compared between the two groups using Student’s t test.

Results : Hydrogels (Gel + Blood group) maintained > 90% mean light transmission from the onset to day 7 of the experimental period. The mean light transmission at each concentration in the Blood group stayed at < 20% until day 7. Mean light transmission of hydrogel (Gel + Blood group) was statistically higher than diluted blood (Blood group) throughout the 28-day experimental period in the samples with 0.25, 0.5, and 1.25% diluted blood (p < 0.05).

Conclusions : Non-swelling polyethylene glycol based artificial vitreous hydrogel maintained high optical transparency in the presence of blood through the study period. Injection of artificial hydrogel into the vitreous cavity at the end of surgery might help prevent or mitigate vitreous hemorrhage-associated postoperative visual loss.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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