July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Influence of light and autonomic innervation on growth factor expression in chick choroid
Author Affiliations & Notes
  • Alexandra Kaser-Eichberger
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
    Dept. of Anatomy, Paracelsus Medical University, Salzburg, Austria
  • Christian Platzl
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
    Dept. of Anatomy, Paracelsus Medical University, Salzburg, Austria
  • Christopher Taylor
    Dept of Biomedical Sciences and Disease, New England College of Optometry, Boston, Massachusetts, United States
  • Andrea Trost
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
  • Clemens Strohmaier
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
  • Barbara Bogner
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
  • Christian Runge
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
  • Daniela Bruckner
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
  • Herbert Reitsamer
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
  • Frances J Rucker
    Dept of Biomedical Sciences and Disease, New England College of Optometry, Boston, Massachusetts, United States
  • Falk Schroedl
    Dept. of Ophthalmology and Optometry, Research Program Experimental Ophthalmology and Glaucoma Research, Paracelsus Medical University, Salzburg, Austria
    Dept. of Anatomy, Paracelsus Medical University, Salzburg, Austria
  • Footnotes
    Commercial Relationships   Alexandra Kaser-Eichberger, None; Christian Platzl, None; Christopher Taylor, None; Andrea Trost, None; Clemens Strohmaier, None; Barbara Bogner, None; Christian Runge, None; Daniela Bruckner, None; Herbert Reitsamer, None; Frances Rucker, None; Falk Schroedl, None
  • Footnotes
    Support  PMU-FFF E-17/25/133-SKE
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5880. doi:
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    • Get Citation

      Alexandra Kaser-Eichberger, Christian Platzl, Christopher Taylor, Andrea Trost, Clemens Strohmaier, Barbara Bogner, Christian Runge, Daniela Bruckner, Herbert Reitsamer, Frances J Rucker, Falk Schroedl; Influence of light and autonomic innervation on growth factor expression in chick choroid. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5880.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Emmetropization is guided by visual input leading to changes in ocular growth rates. Unknown retinal growth signals are transferred into the sclera, with the choroid as an intermediary. Growth regulation depends on growth factor expression, but also on vascular supply, and hence on choroidal blood flow that is regulated by the autonomic nervous system. While light stimulation alters choroidal blood flow, we ask if different light stimuli influence choroidal growth factor expression and we tested for the role of the autonomic nervous system in this context.

Methods : White Leghorns (1 to 2 weeks old) received unilateral lesion of the ocular parasympathetic (ciliary/pterygopalatine ganglion; n=20) or sympathetic (superior cervical ganglion; n=26) pathways. Following recovery (1 week), chicks were exposed to steady yellow light (y; n=10; mean illumination 680 lux) or sinusoidal modulated light (2Hz: 80% contrast) that changed in luminance contrast (yellow to black; yk; n=19) or color contrast (yellow to blue; yb; n=17). Chicks were kept in these conditions (3 days, 9am-5pm; otherwise in dark) and sacrificed immediately after light cycle. Choroids (n≥5/group) were removed and prepared for quantitative RT-PCR of BMP2, IGF1, EGF, PDGFA, PDGFB, PDGFC, TGFB1, TGFB2, TGFB3, VEGFA, VEGFC. Relative gene expression levels were determined (normalized to HPRT) followed by statistical analysis (t-test or Wilcoxon test; ANOVA) to compare lesioned vs non-lesioned eyes.

Results : In parasympathetic lesion, EGF, IGF1, and TGFB1 were up-regulated (p≤0.05) in at least one light condition (y: EGF, IGF1, TGFB1; yk: IGF1; yb: EGF, IGF1). In contrast, in sympathetic lesion, expression of EGF, IGF1, PDGFC, TGFB2, and VEGFA was down-regulated (p≤0.05); however, this effect occurred in flicker conditions only (yk: EGF, IGF1, TGFB2, VEGFA; yb: EGF, PDGFC, TGFB2, VEGFA), but not in steady light (y). No change in any condition (lesion and light) was seen for BMP2, PDGFA/B, TGFB3, and VEGFC.

Conclusions : In response to different light conditions, lesion of ocular autonomic innervation changed expression of choroidal growth factors. Effects differed in sympathetic and parasympathetic lesions. More extensive expression analysis will be necessary to link these results with physiological events like light-induced changes in choroidal blood flow and to clarify the role of the autonomic nervous system in light-induced ocular growth changes.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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