July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Short-wavelength and near-Infrared fundus autofluorescence in blue light-illuminated Abca4-/- mice and a patient with Stargardt disease
Author Affiliations & Notes
  • Yuan Fang
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
    STZ OcuTox Preclinical Drug Assesment, Hechingen, Germany
  • Alexander Tschulakow
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
    STZ OcuTox Preclinical Drug Assesment, Hechingen, Germany
  • Tatjana Taubitz
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
  • Barbara Illing
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
  • Antonina Burda
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
  • Antje Biesemeier
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
    Natural and Medical Institute at the University of Tuebingen, Applied Material Science and Electron Microscopy, Germany
  • Sylvie Julien
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
  • Ulrich Schraermeyer
    Center for Ophthalmology, Division for experimental vitreoretinal surgery, Tuebingen, Germany
    STZ OcuTox Preclinical Drug Assesment, Hechingen, Germany
  • Footnotes
    Commercial Relationships   Yuan Fang, None; Alexander Tschulakow, None; Tatjana Taubitz, None; Barbara Illing, None; Antonina Burda, None; Antje Biesemeier, None; Sylvie Julien, None; Ulrich Schraermeyer, None
  • Footnotes
    Support  China Scholarship Council
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5982. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Yuan Fang, Alexander Tschulakow, Tatjana Taubitz, Barbara Illing, Antonina Burda, Antje Biesemeier, Sylvie Julien, Ulrich Schraermeyer; Short-wavelength and near-Infrared fundus autofluorescence in blue light-illuminated Abca4-/- mice and a patient with Stargardt disease. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5982.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Fundus autofluorescence imaging is a noninvasive technique for diagnosing retinopathies, such as Stargardt disease (SD). The interpretation of short- wavelength (SW) fundus autofluorescence (AF) and near-infrared (NIR) AF alterations of retina disease is not fully studied.To determine histological features of aberrances in SW-AF and NIR-AF, we investigated blue light-illuminated Abca4-/- mice and perimacular tissue from a donor with Stargardt disease.

Methods : Pigmented Abca4−/− mice (129S4/SvJae-Abca4tm1Ght) were illuminated with blue light (430nm, 50mW/cm2, 15 min). Fundus AF in modalities of SW-AF and NIR-AF and spectral-domain optical coherence tomography (SD-OCT) were recorded 7 days after blue light illumination. Semithin sections were observed under light microscope and fluorescence microscope (filter for lipofuscin: excitation 360 nm, emission 540 nm; filter for NIR fluorescence: excitation 750 nm, emission 773nm). Ultrastructure was analyzed by electron microscope. Fundus AF images were correlated to histological analysis. Semithin sections of a 72-year-old donor with Stargardt disease were examined under fluorescence microscope with both filters.

Results : In blue light-illuminated Abca4−/− mice, patches with fundus hyper AF in both modalities of SW-AF and NIR-AF correlated with hyperreflective bands in OCT and appeared as accumulation of high number of macrophages in the subretinal space. Macrophages were observed with hyperAF under fluorescence microscope with both filters. Lesion areas of complete loss of RPE and photoreceptors were observed with loss of AF. In the human donor eye with late Stargardt disease, hyperAF of lipofuscin was observed in RPE cells. The prominent cluster with lipofuscin AF in RPE layer as shown earlier (Taubitz et al (2018). PeerJ 6: e5215) exhibited extremely hyper NIR-AF under fluorescence microscope. Photoreceptors were not observed with lipofuscin-AF or NIR-AF.

Conclusions : Accumulation of macrophages which phagocytose the debris containing high concentrations of melanosomes, melanolipofuscin and lipofuscin granules results in fundus hyperAF in modalities of SW-AF and NIR-AF in blue light-illuminated pigmented Abca4-/- mice. Blue light induced geographic atrophy results in loss of AF. In eye with Stargardt disease, the pigment cluster expresses high NIR-AF inidicating changes in the chemical compositions of melanin.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×