July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Growth hormone-releasing hormone receptor signaling in rat retinal development
Author Affiliations & Notes
  • Qichen Yang
    Ophthalmology and Visual Sciences, the Chinese University of Hong Kong, Hong Kong, Hong Kong
  • Chi Chui Wang
    Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong
  • Sun On Chan
    School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong
  • Herman S Cheung
    Geriatric Research, Miami Veterans Affairs Medical Center, Florida, United States
    Biomedical Engineering, University of Miami, Florida, United States
  • Andrew V Schally
    Endocrine, Polypeptide and Cancer Institute, Veterans Affairs Medical Center, Florida, United States
    Department of Endocrinology, University of Miami, Florida, United States
  • Calvin C P Pang
    Ophthalmology and Visual Sciences, the Chinese University of Hong Kong, Hong Kong, Hong Kong
    Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, China
  • Tsz Kin Ng
    Ophthalmology and Visual Sciences, the Chinese University of Hong Kong, Hong Kong, Hong Kong
    Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, China
  • Footnotes
    Commercial Relationships   Qichen Yang, None; Chi Chui Wang, None; Sun On Chan, None; Herman S Cheung, None; Andrew V Schally, None; Calvin Pang, None; Tsz Kin Ng, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 6012. doi:
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    • Get Citation

      Qichen Yang, Chi Chui Wang, Sun On Chan, Herman S Cheung, Andrew V Schally, Calvin C P Pang, Tsz Kin Ng; Growth hormone-releasing hormone receptor signaling in rat retinal development. Invest. Ophthalmol. Vis. Sci. 2019;60(9):6012.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We previously demonstrated that growth hormone-releasing hormone (GHRH) receptor is expressed in rat adult retina and participated in uveitis and retinoblastoma development. Here we aimed to investigate the GHRH receptor signaling in rat retinal development and retinal progenitor cell differentiation.

Methods : GHRH receptor expression was determined in the retinas of Sprague-Dawley rats (embryonic day (E) 10 to adult) by the immunofluorescence analysis. Retinal progenitor cells from E18 and post-natal day (PN) 1 rat retinas were isolated, purified and cultured in DMEM/F-12 medium with N2 supplement, basic fibroblast growth factor and epidermal growth factor on the non-adherent culture dishes for 3 days, followed by the treatment of retinal differentiation medium (DMEM/F-12 medium with B27 supplement) with the GHRH agonist and antagonist for 7 days. Retinal cell differentiation was evaluated by the immunofluorescence analysis of specific retinal cell markers (Chx10 for retinal progenitor cells, TuJI for retinal ganglion cells, rhodopsin for photoreceptors and GFAP for Müller glia).

Results : In rat retina, the GHRH receptor was expressed in the retinal ganglion cell layer from E14 to adult. During retinal progenitor cell differentiation, the GHRH receptor agonist and antagonist both upregulated the expression of GHRH receptor in the E18 and PN1 induced retinal progenitor cells, compared to the vehicle control group. Upon GHRH receptor antagonist treatment, the expression of Chx10 in the induced retinal progenitor cells was decreased, whereas the expressions of GFAP and rhodopsin were increased, compared to the vehicle control groups. Upon GHRH receptor agonist treatment, Chx10 and rhodopsin expressions were increased in the induced retinal progenitor cells, whereas the expression of GFAP was decreased. There was no significant difference in TuJI expression among the three treatment groups.

Conclusions : GHRH receptor is expressed in retinal ganglion cell layer along the rat retinal development. GHRH receptor signaling could be involved in retinal progenitor maintenance as well as photoreceptor and Müller glia differentiation.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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