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Wenting Cai, Jing Yu; Fluorescein sodium loaded by polyethyleneimine for fundus fluorescein angiography. Invest. Ophthalmol. Vis. Sci. 2019;60(9):6101.
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To develope nanoparticles with good biosecurity and low retention for fundus angiography.
Polyethyleneimine (PEI) used as a template was modified with fluorescein sodium (FS), and after acetylation, PEI-NHAc-FS NPs was developed for fundus angiography. PEI-NHAc-FS NPs with a mean diameter of 181.33nm was chelated with fluorescein sodium. PEI-NHAc-FS were formed and characterized by ZP, DLS, 1H NMR, UV and DTA-TG. In vitro biocompatibility of PEI-NHAc-FS NPs was performed in ARPE-19 cells and HUVECs and was evaluated by different methods. The cytotoxicity of FS and PEI-NHAc-FS NPs were determined via CCK-8 assay; cellular apoptosis was evaluated by flow cytometry assay, and the cell morphology was observed with a light microscope. Angiogenesis were examined via tube formation assay. In vitro cellular uptake was determined by flow cytometer using argon-ion 488-nm lasers and a fluorescence microscope. Laser photocoagulation-induced CNV rat models were established. Through intravenous tail injection, normal and CNV rats were injected with 0.5%, 1%, 5% FS and PEI-NHAc-FS. Fundus fluorescein angiography was applied to record the fluorescein images. Electroretinogram and HE staining of organs were performed to observe biocompatibility in vivo.
In our study, PEI-NHAc-FS NPs has good biocompatibility in vitro and in vivo. There are no cellular toxicity, apoptosis, morphology and tube formation in the studied concentration range in vitro; meanwhile, intravenous injection of the designed NPs did not cause functional damage to the retina, and no histological damage is observed in the organs, including heart, liver, spleen, kidney, brain and muscle in vivo. PEI-NHAc-FS can reduce cellular uptake in vitro and thus speed the metabolism in ocular vessels. The designed NPs can also develop normal retinal vessels and CNV leakages effectively, and they can leave the retina faster than FS, thereby reducing the duration of fundus vessel exposure.
In a nut shell, we develop a smart nanosize fluorescence contrast agents, the preparation method is simple and this study hold great potential for the clinical FFA diagnosis, therapy and research work.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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