July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
The correlation between retinal pigment epithelium thickness and lipofuscin accumulation in healthy eyes: an in vivo optical coherence tomography and quantitative autofluorescence study
Author Affiliations & Notes
  • Mariano Cozzi
    Ophthalmology, Eye Clinic Luigi Sacco Hospital, University of Milan, Milan, Italy
  • Maria Belotti
    Ophthalmology, Eye Clinic Luigi Sacco Hospital, University of Milan, Milan, Italy
  • Mario Cigada
    Ophthalmology, Eye Clinic Luigi Sacco Hospital, University of Milan, Milan, Italy
  • Francesco Viola
    Ophthalmology, IRCCS-Cà Granda Foundation- Ospedale Maggiore Policlinico, University of Milan, Milan, Italy
  • Giovanni Staurenghi
    Ophthalmology, Eye Clinic Luigi Sacco Hospital, University of Milan, Milan, Italy
  • Alessandro Invernizzi
    Ophthalmology, Eye Clinic Luigi Sacco Hospital, University of Milan, Milan, Italy
  • Footnotes
    Commercial Relationships   Mariano Cozzi, Carl Zeiss Meditec Inc. (R), CenterVue (F); Maria Belotti, None; Mario Cigada, None; Francesco Viola, Allergan (F), Bayer (F), Novartis (F), Roche (F), SIFI (F); Giovanni Staurenghi, Allergan (C), Appellis (C), Bayer (C), Boheringer (C), Carl Zeiss Meditec (C), CenterVue (C), CenterVue (F), Genetech (C), Heidelberg Engineering (C), Heidelberg Engineering (F), Heidelberg Engineering (R), Novartis (C), Novartis (F), Ocular Instruments (P), Optos (F), OptoVue (F), Quantel Medical (C), Quantel Medical (F), Roche (C), Topcon (F); Alessandro Invernizzi, Allergan (R)
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 6102. doi:
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      Mariano Cozzi, Maria Belotti, Mario Cigada, Francesco Viola, Giovanni Staurenghi, Alessandro Invernizzi; The correlation between retinal pigment epithelium thickness and lipofuscin accumulation in healthy eyes: an in vivo optical coherence tomography and quantitative autofluorescence study. Invest. Ophthalmol. Vis. Sci. 2019;60(9):6102.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : age related lipofuscin accumulation within retinal pigment epithelium (RPE) cells has been demonstrated in healthy subjects in vivo using quantitative fundus autofluorescence (qAF). The aim of this study was to investigate the possible correlation existing between the RPE thickness and the qAF values in a healthy Caucasian population.

Methods : Healthy volunteers between 18 and 65 years of age were enrolled. All subjects underwent Spectral Domain Optical Coherence Tomography (SD-OCT) and qAF (Heidelberg Spectralis) imaging. SD-OCT images were analyzed using the built-in graph-based automatic segmentation algorithm for single retinal layers identification to assess RPE thickness. Only values within central and overall intermediate ETDRS grid were considered for the analysis. Using a customized dedicated software, qAF images were also evaluated and values of eight subfields forming a ring centered onto the fovea were collected and averaged in order to obtain a single qAF value (qAF8).
The correlation between the RPE thickness (RPEt) in the central and intermediate ETDRS ring and the age of the subjects was assessed. The effect of qAF and age on the RPEt was studied with a multivariate regression analysis.

Results : A total of 105 eyes from 105 subjects (mean age 42.1±13.9 years) were included in the analysis. The RPEt significantly increased with age in the intermediate ETDRS ring (r=0.29,P<0.05). The values of qAF were also positively correlated with age (r=0.68,P<0.05). A significant correlation was found between qAF and RPEt, However, after adjusting for age in the multivariate regression model, the correlation remained significant only for subjects younger than 40 years of age (P<0.05).

Conclusions : The RPEt increased with age in a healthy population. A similar increase was found for qAF values. After adjusting for age, qAF and RPEt were still correlated in subjects younger than 40 years. Since qAF indirectly measures the lipofuscin amount within the RPE, the thickening of the RPE measured in vivo with OCT is likely correlated with lipofuscin accumulation. These findings could help in understanding the pathophysiology of a variety of diseases determined by lipofuscin damage to the RPE.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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