July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Plasma Rich in Growth Factors membrane in the treatment of Limbal Stem Cell Deficiency.
Author Affiliations & Notes
  • Jesus Merayo-Lloves
    Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica & Universidad de Oviedo, Oviedo, Asturias, Spain
  • Ronald Sanchez-Avila
    Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica & Universidad de Oviedo, Oviedo, Asturias, Spain
  • Manuel Chacon
    Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica & Universidad de Oviedo, Oviedo, Asturias, Spain
  • Natalia Vazquez
    Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica & Universidad de Oviedo, Oviedo, Asturias, Spain
  • Silvia Berisa
    Instituto Oftalmologico Fernandez-Vega, Spain
  • MAIROBI PERSINAL MEDINA
    Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica & Universidad de Oviedo, Oviedo, Asturias, Spain
  • Carlos Lisa
    Instituto Oftalmologico Fernandez-Vega, Spain
  • Miguel Naveiras
    Instituto Oftalmologico Fernandez-Vega, Spain
  • Alvaro Meana
    Instituto Universitario Fernandez-Vega, Fundacion de Investigacion Oftalmologica & Universidad de Oviedo, Oviedo, Asturias, Spain
  • Footnotes
    Commercial Relationships   Jesus Merayo-Lloves, None; Ronald Sanchez-Avila, None; Manuel Chacon, None; Natalia Vazquez, None; Silvia Berisa, None; MAIROBI MEDINA, None; Carlos Lisa, None; Miguel Naveiras, None; Alvaro Meana, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 6314. doi:
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      Jesus Merayo-Lloves, Ronald Sanchez-Avila, Manuel Chacon, Natalia Vazquez, Silvia Berisa, MAIROBI PERSINAL MEDINA, Carlos Lisa, Miguel Naveiras, Alvaro Meana; Plasma Rich in Growth Factors membrane in the treatment of Limbal Stem Cell Deficiency.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):6314.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To prove the efficacy of a Plasma Rich in Growth Factors (PRGF) membrane for the treatment of a rabbit alkali-burn induced Limbal Stem Cell Deficiency (LSCD).

Methods : A total LSCD was induced in 12 healthy male New Zealand rabbits by applying a gauze soaked in 1M NaOH for 10 seconds. A week later, clinical manifestations of LSCD (epithelial integrity, corneal neovascularization, fibrovascular pannus and corneal opacity) were evaluated and rabbits were diagnosed and treated. Animals were randomly divided into four groups of three rabbits each: PRGF membrane cultured with autologous or heterologous rabbit LEPCs, PRGF membrane with autologous Simple Limbal Epithelial Transplantation (SLET) and PRGF membrane only. Once a week, clinical manifestations of LSCD were evaluated. After 40 days of clinical follow-up, all rabbits were euthanized and excised corneas were subjected to histochemical examination with hematoxylin-eosin and immunostaining against Cytokeratin 3/12 to identify differentiated corneal epithelial cells and against MUC1, MUC5AC and Cytokeratin 7 to identify conjunctival epithelial cells.

Results : Seven days after alkali-burn lesion, all rabbits showed a total LSCD with opaque cornea, new blood vessels across the limbus penetrating into the cornea, and epithelial defects shown by fluorescein stain. At the end of the follow-up period an improvement of the four clinical parameters analyzed was observed in all the rabbits. However, only rabbits transplanted with cultured LEPCs had positive CK3-12 stain and negative stain against CK7, MUC1 and MUC5AC, indicating the presence of a normal corneal epithelium (with the exception of one rabbit in the group 1). Otherwise, rabbits transplanted with PRGF membrane with autologous SLET or with only PRFG membrane showed positive stain against CK7, negative stain against CK3-12 and, in some cases, positive stain against MUC1 and MUC5AC, indicating the presence of a conjunctival epithelium with or without goblet cells.

Conclusions : PRGF membrane improved the experimental chemically-induced lesions. Nonetheless, only PRGF membranes with cultured LEPCs were able to restore the corneal surface.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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