July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Expression and functional analysis of Wnt/PCP components in tear drainage system
Author Affiliations & Notes
  • Jiali Ru
    Zhongshan ophthalmic center, Guangzhou, China
  • Dianlei Guo
    Zhongshan ophthalmic center, Guangzhou, China
  • Chunqiao Liu
    Zhongshan ophthalmic center, Guangzhou, China
  • Footnotes
    Commercial Relationships   Jiali Ru, None; Dianlei Guo, None; Chunqiao Liu, None
  • Footnotes
    Support  Guangzhou City Sciences and echnologies Innovation Project (201707020009; Guangzhou, Guangdong Province, China), the National Natural Science Foundation of China (NSFC: 31571077; Beijing, China) and ‘‘100 People Plan’’ from Sun Yat-sen University (8300-18821104; Guangzhou, Guangdong Province, China) to Chunqiao Liu; by grant from Science and Technology Planning Project of Guangdong Province (No. 2015B020226003) to Hong Ouyang
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 6383. doi:
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      Jiali Ru, Dianlei Guo, Chunqiao Liu; Expression and functional analysis of Wnt/PCP components in tear drainage system. Invest. Ophthalmol. Vis. Sci. 2019;60(9):6383.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Ocular drainage system is essential for tear flow and eye sanitation. Our study aims to illustrate the role of Wnt/PCP signaling in tear duct development.

Methods : In situ hybridization and immunohistochemistry were conducted to examine expression of Wnt/PCP components during tear duct initiation and elongation. H&E histology was used to examine tear duct anatomy. Genetically engineered Prickle mutant mice were used for functional analysis of PCP signaling.

Results : All 19 Wnt genes and PCP core components including Frizzled, Prickle, Celsr, Vangl, Dishevelled and Inversin genes were examined. A subset of Wnt/PCP components were expressed in developmental tear duct, suggesting a requirement of Wnt/PCP signaling for tear drainage system development. Particularly, Prickle 1 is largely restricted to the nasolacrimal duct and canaliculi. We further demonstrated that PCP signaling is essential for development of tear drainage system using Prickle 1 mutant mouse model.

Conclusions : We concluded that Wnt/PCP signaling plays an essential role in tear drainage system develpoment. Ongoing analysis is elucidating molecular mechanisms of Wnt/PCP signaling-driven tear duct development.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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