Abstract
Purpose :
Previous studies have reported molecular biological changes of the vitreous in various vitreoretinal diseases. Our purpose was to explore the immunological components that are responsible for the proliferative alterations in different forms of retinal detachment.
Methods :
Vitreous samples were collected during 23G pars plana vitrectomy from 54 eyes of 54 patients with retinal detachment: rhegmatogenous retinal detachment without proliferative retinopathy (PVR) (n=30), with PVR (n=16), proliferative diabetic retinopathy with tractional retinal detachment (n=8). Vitreous fluid was obtained from 19 eyes with epiretinal membrane as controls. A multiplex chemiluminescent immunoassay was performed to evaluate the concentrations of 48 cytokines, chemokines and growth factors (Bio-Plex beads array kit, Bio-Rad Laboratories, Hercules, CA). Statistical analysis (Kruskal-Wallis test, P<0.05) was performed to asses the differences between the groups.
Results :
Levels of IL-6, IL-8, IL-16, IFN-gamma, MIP-1beta, MCP-1, MIF and eotaxin were significantly higher in all groups of retinal detachment compared to the group of epiretinal membrane. Levels of CTACK, IP-10, SCGF-beta and SDF-1alpha were significantly higher in patients with tractional retinal detachment and PVR. Levels of IL-18 and VEGF were significantly higher in tractional retinal detachment.
Conclusions :
Our results indicate that there are complex immunological changes in different forms of retinal detachment depending on the level of proliferation. In the future these findings may serve as biomarkers of proliferation and may help to invent therapeutic strategies to prevent these pathological changes.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.