July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Effects of Probucol on high glucose-induced expressions of SP1/Keap1/Nrf2/GCLC in the cultured human Müller cells
Author Affiliations & Notes
  • Zhongping CHEN
    AIER School of Ophthalmology, Central South University, Changsha, Hunan Province, China
    Department of Fundus Oculi, AIER Eye Hospital of Changsha, Changsha, Hunan Province, China
  • Chenxiang Li
    AIER School of Ophthalmology, Central South University, Changsha, Hunan Province, China
  • Shibei Ai
    Department of Ophthalmology, the Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong Province, China
  • Xuxia Zhou
    AIER School of Ophthalmology, Central South University, Changsha, Hunan Province, China
  • Footnotes
    Commercial Relationships   Zhongping CHEN, None; Chenxiang Li, None; Shibei Ai, None; Xuxia Zhou, None
  • Footnotes
    Support  Natural Science Foundation Project of Hunan Province (No.2018JJ2001); Research Fund Project of AIER Eye Hospital Group (No.AF1601D5)
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 5368. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Zhongping CHEN, Chenxiang Li, Shibei Ai, Xuxia Zhou; Effects of Probucol on high glucose-induced expressions of SP1/Keap1/Nrf2/GCLC in the cultured human Müller cells. Invest. Ophthalmol. Vis. Sci. 2019;60(9):5368.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The aim of this study is to investigate the effects of Probucol on high glucose-induced expressions of specificity protein 1(SP1), kelchlike ECH associated protein1 (Keap1), NF-E2-related factor 2 (Nrf2) and glutamate-cysteine ligase catalytic (GCLC) in the cultured human Müller cells and explore the protective role of the Probucol on Müller cells in high glucose environment.

Methods : Müller cells were cultured from human retinal tissue, and identified by their expression of glutamine synthetase(GS). The cells were randomly divided into four groups: normal glucose group (group A); normal glucose with Probucol (group B); high glucose group (group C); high glucose with Probucol (group D). The expression of SP1, Keap1, Nrf2, and GCLC was examined by immunocytochemitry. The messenger RNA (mRNA) expressions of SP1, Keap1, Nrf2, and GCLC were evaluated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).

Results : More than 95% of the cultured cells expressed GS. Immunocytochemistry showed that SP1, Keap1, Nrf2, and GCLC proteins were all expressed by human Müller cells. qRT-PCR indicated that SP1, Keap1, and Nrf2 mRNAs in group C increased significantly compared with group A(P<0.05)and GCLC mRNA in group C decreased significantly compared with group A(P<0.050). However, SP1 and Keap1 mRNAs in group D decreased significantly compared with group C(P<0.05), Nrf2 and GCLC mRNAs in group D increased compared with group C(P<0.05).

Conclusions : The expressions of SP1, Keap1, and Nrf2 were up-regulated, while the expression of GCLC was down-regulated by high glucose in cultured human Müller cells. Probucol can mitigate such effects of high glucose.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

The result of human müller cells immunofluorescence staining:A. The positive results of GCLC expression were green, scaleplate:10um;B. The positive results of GS expression were red, scaleplate:10um;C.The positive result of nuclear DAPI expression was blue, scaleplate:10um;D.A+B+C,scaleplate:10um;E. The positive results of Nrf2 expression were green, scaleplate:10um;F. The positive results of Keap1 expression were red, scaleplate:10um;G. The positive result of nuclear DAPI expression was blue, scaleplate:10um;H.E+F+G, scaleplate:10um

The result of human müller cells immunofluorescence staining:A. The positive results of GCLC expression were green, scaleplate:10um;B. The positive results of GS expression were red, scaleplate:10um;C.The positive result of nuclear DAPI expression was blue, scaleplate:10um;D.A+B+C,scaleplate:10um;E. The positive results of Nrf2 expression were green, scaleplate:10um;F. The positive results of Keap1 expression were red, scaleplate:10um;G. The positive result of nuclear DAPI expression was blue, scaleplate:10um;H.E+F+G, scaleplate:10um

 

I. The positive results of SP1 expression were red, scaleplate:15um;J. The positive result of nuclear DAPI expression was blue, scaleplate:15um;K.I+J, scaleplate:15um.

I. The positive results of SP1 expression were red, scaleplate:15um;J. The positive result of nuclear DAPI expression was blue, scaleplate:15um;K.I+J, scaleplate:15um.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×