July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Comparison of the therapeutic effects between topical 8-oxo-2'-deoxyguanosine and steroid in ocular chemical burn experimental model
Author Affiliations & Notes
  • Dong Hyun Kim
    Ophghalmology, Gachon University College of Medicine, Incheon, Korea (the Republic of)
  • Sang-Taek Im
    Gachon Biomedical & Convergence Institute, Korea (the Republic of)
  • Sang Beom Han
    Ophghalmology, Department of Ophthalmology, Kangwon National University College of Medicine, Korea (the Republic of)
  • Myung-Hee Chung
    Gachon Biomedical & Convergence Institute, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Dong Hyun Kim, None; Sang-Taek Im, None; Sang Beom Han, None; Myung-Hee Chung, None
  • Footnotes
    Support  This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science and ICT (2017R1C1B5017111), and Gil Medical Center (Project No. FRD2015-04-02).
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 886. doi:
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    • Get Citation

      Dong Hyun Kim, Sang-Taek Im, Sang Beom Han, Myung-Hee Chung; Comparison of the therapeutic effects between topical 8-oxo-2'-deoxyguanosine and steroid in ocular chemical burn experimental model. Invest. Ophthalmol. Vis. Sci. 2019;60(9):886.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare the effects of topical 8-oxo-dG and steroids on ocular chemical burn mice model

Methods : We created ocular chemical burn models with 8-week-old BALB/c mice (n=45) by applying 0.1M sodium hydroxide(NaOH); the mice were then treated with 8-oxo-dG eye drops 10 mg/ml, 0.1% fluorometholone ophthalmic suspension, and phosphate buffered saline (PBS) twice daily. After seven days, clinical findings such as corneal integrity, clarity, and neovascularization were assessed. Histology, immunohistochemistry, inflammatory cytokine levels using real time polymerase chain reactions (PCRs), and amount of total reactive oxygen species in the corneas of the mice were also compared.

Results : Topical application of 8-oxo-dG and steroid resulted in a significant improvement of epithelial defects and clarity, compared to PBS (each p<0.05). Stromal thickness in cornea was also decreased in the 8-oxo-dG and steroid treated mice, compared to PBS-treated controls. (p<0.001) The expressions of F4/80 and neutrophil elastase-positive inflammatory cells in the stroma, and IL-1β and IL-6 cytokine levels in whole cornea were significantly reduced in the 8-oxo-dG group, compared to PBS group (each p<0.05).

Conclusions : In the ocular chemical burn experimental model, topical 8-oxo-dG showed excellent anti-inflammatory effects those were not inferior than topical steroids.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

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