July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Ultrahigh-resolution anterior segment optical coherence tomography in corneal wound healing
Author Affiliations & Notes
  • Rene Marcel Werkmeister
    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Vienna, Austria
    Christian Doppler Laboratory of Ocular and Dermal Effects of Thiomers, Medical University of Vienna, Vienna, Austria
  • Anca Pantalon
    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Vienna, Austria
    Department of Ophthalmology, Gr. T. Popa University of Medicine and Pharmacy, Iasi, Romania
  • Martin Pfister
    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Vienna, Austria
    Christian Doppler Laboratory of Ocular and Dermal Effects of Thiomers, Medical University of Vienna, Vienna, Austria
  • Valentin Aranha dos Santos
    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Vienna, Austria
  • Gerald Schmidinger
    Department of Ophthalmology, Medical University of Vienna, Vienna, Austria
  • Gerhard Garhofer
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
  • Doreen Schmidl
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
  • Leopold Schmetterer
    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Vienna, Austria
    Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore
  • Footnotes
    Commercial Relationships   Rene Werkmeister, None; Anca Pantalon, None; Martin Pfister, None; Valentin Aranha dos Santos, None; Gerald Schmidinger, None; Gerhard Garhofer, None; Doreen Schmidl, None; Leopold Schmetterer, None
  • Footnotes
    Support  Christian Doppler Research Association, the Austrian Federal Ministry of Digital and Economic Affairs and the National Foundation of Research, Technology and Development; Hochschuljubiläumsstiftung der Stadt Wien (project H-289408/2013)
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 3219. doi:
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    • Get Citation

      Rene Marcel Werkmeister, Anca Pantalon, Martin Pfister, Valentin Aranha dos Santos, Gerald Schmidinger, Gerhard Garhofer, Doreen Schmidl, Leopold Schmetterer; Ultrahigh-resolution anterior segment optical coherence tomography in corneal wound healing. Invest. Ophthalmol. Vis. Sci. 2019;60(9):3219.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate whether ultrahigh-resolution OCT (UHR-OCT) is a suitable method for the in-vivo evaluation and follow up of corneal epithelial wound healing.

Methods : Corneal epithelial wound healing was imaged in human keratoconic corneas after epi-off crosslinking (CXL) procedure and in rabbits with a standardized model of corneal injury by means of a custom-built UHR-OCT system. Volumetric OCT data sets provided epithelial thickness maps and allowed for evaluation of the stage of reepithelization. In addition, accompanying changes in deeper corneal microarchitecture were analyzed.

Results : A total of 40 keratoconic eyes were included in the human study. The mean central corneal thickness at baseline before CXL was 482.7 ± 38.2 µm with a mean central epithelial thickness (CET) of 43.8 µm ± 6.37 µm. 20 ± 5 days after the CXL procedure, where clinical examination via slitlamp biomicroscopy and fluorescein staining revealed complete wound closure, CET was 35.0 µm ± 5.8 µm, significantly thinner than before CLX (p<0.001). The demarcation line measured at this time point was identified at 43.7 ± 13.5 % stromal depth.
In rabbits, the mean CET in 20 eyes at baseline was 35.9 ± 2.6 µm. In animals with complete wound closure (10/20 eyes) at the last study day at 72 hours, CET was significantly thinner as compared to baseline (30.4 ± 2.8 µm vs. 35.4 ± 2.9 µm, p = 0.005), while in rabbits with incomplete wound closure, wound area was still 27.4 ± 13.8 % of the initial value. An intra-stromal landmark indicating early keratocyte apoptosis triggered by the epithelial abrasion in was measured at 30.0 ± 5.1 % stromal depth.

Conclusions : UHR-OCT provided 3D assessment of the time course of epithelial wound healing by means of epithelial thickness mapping in human and in rabbit corneas. This technique may in future allow for a better understanding of corneal epithelial wound healing and for the testing of new therapeutic approaches to improve reepithelization in chronic corneal epithelial defects.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

UHR-OCT image of a patient 15 days after CXL procedure revealing the stromal depth of the demarcation line.

UHR-OCT image of a patient 15 days after CXL procedure revealing the stromal depth of the demarcation line.

 

Corneal imaging in rabbits. (b) UHR-OCT and (c) histology specimen with H&E staining of the cornea of a New Zealand White rabbit. Non-invasive in vivo imaging visualizes all corneal layers: EP, epithelium; BLE, basal layer of epithelium; BL, Bowman’s layer; ST, corneal stroma; DM, Descemet’s membrane; ED, endothelium.

Corneal imaging in rabbits. (b) UHR-OCT and (c) histology specimen with H&E staining of the cornea of a New Zealand White rabbit. Non-invasive in vivo imaging visualizes all corneal layers: EP, epithelium; BLE, basal layer of epithelium; BL, Bowman’s layer; ST, corneal stroma; DM, Descemet’s membrane; ED, endothelium.

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