July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Intra- and Inter-Subject Variability of Retinal Oximetry on Healthy Eyes Using Visible-Light OCT
Author Affiliations & Notes
  • Zeinab Ghassabi
    Ophthalmology, NYU Langone Health, NYU Eye Center, New York, NY, New York, New York, United States
  • Katie Lucy
    Ophthalmology, NYU Langone Health, NYU Eye Center, New York, NY, New York, New York, United States
  • Mengfei Wu
    Ophthalmology, NYU Langone Health, NYU Eye Center, New York, NY, New York, New York, United States
    Division of Biostatistics, Departments of Population Health and Environmental Medicine, NYU School of Medicine, New York, NY., New York, United States
  • Gadi Wollstein
    Ophthalmology, NYU Langone Health, NYU Eye Center, New York, NY, New York, New York, United States
  • Joel S Schuman
    Ophthalmology, NYU Langone Health, NYU Eye Center, New York, NY, New York, New York, United States
  • Brian Soetinko
    Department of Biomedical Engineering, Northwestern University, Evanston IL 60208, Louisiana, United States
  • Yuanbo Wang
    Opticent Inc. Evanston IL 60208, New York, United States
  • Roman Kuranov
    Opticent Inc. Evanston IL 60208, New York, United States
    Department of Biomedical Engineering, Northwestern University, Evanston IL 60208, Louisiana, United States
  • Hao F Zhang
    Department of Biomedical Engineering, Northwestern University, Evanston IL 60208, Louisiana, United States
  • Hiroshi Ishikawa
    Ophthalmology, NYU Langone Health, NYU Eye Center, New York, NY, New York, New York, United States
  • Footnotes
    Commercial Relationships   Zeinab Ghassabi, None; Katie Lucy, None; Mengfei Wu, None; Gadi Wollstein, None; Joel Schuman, Zeiss (P); Brian Soetinko, None; Yuanbo Wang, Opticent Inc. (E); Roman Kuranov, Opticent Inc. (E); Hao Zhang, Opticent Inc. (I), Opticent Inc. (P); Hiroshi Ishikawa, None
  • Footnotes
    Support  NIH: R01-EY013178
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1287. doi:https://doi.org/
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    • Get Citation

      Zeinab Ghassabi, Katie Lucy, Mengfei Wu, Gadi Wollstein, Joel S Schuman, Brian Soetinko, Yuanbo Wang, Roman Kuranov, Hao F Zhang, Hiroshi Ishikawa; Intra- and Inter-Subject Variability of Retinal Oximetry on Healthy Eyes Using Visible-Light OCT. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1287. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate intra- and inter-subject variability of the oxygen saturation (sO2) measurements in major retinal blood vessels using visible-light optical coherence tomography (vis-OCT).

Methods : Four eyes of 4 healthy volunteers were enrolled. For each eye, one pair of retinal major artery and vein in superior and inferior regions near the optic nerve head (Fig.) were repeatedly scanned four times during the same session with our prototype vis-OCT (8192 A-scans over 1mm2, sampling interval = 0.12 µm). Spectroscopic analysis was done on the raster scans using short-time Fourier Transform (STFT). For reliable sO2 estimation, wavelength-dependent OCT amplitude from same depth location across multiple A-lines were averaged. Coefficient of variations (CVs) were calculated to assess sO2 measurement repeatability for each subject/location. Intraclass correlations (ICCs) were calculated to assess inter-subject variability on sO2 difference between the paired artery and vein.

Results : Subjects demographics and average sO2 measurements are shown in Table 1. Intra-subject measurement repeatability was very high (CVs ranging from 0.21 to 1.76%, Table 2). The retinal arteries showed consistently higher sO2 than the paired veins regardless the location. The sO2 differences between the paired artery and vein showed small variability even among subjects (Table 3).

Conclusions : The sO2 measurements showed high intra-subject repeatability and low inter-subject measurement variability. Vis-OCT technology may be a useful and reproducible clinical tool for performing retinal oximetry in vivo.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

 

Figure. Sampling locations of retinal oximetry. A pair of artery (A) and vein (V) in superior(sup) and inferior(inf) regions near the optic nerve head are sampled to measure sO2 (highlighted dashed lines).

Figure. Sampling locations of retinal oximetry. A pair of artery (A) and vein (V) in superior(sup) and inferior(inf) regions near the optic nerve head are sampled to measure sO2 (highlighted dashed lines).

 

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